保川 清

Last Update: 2017/06/15 15:49:40

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Name(Kanji/Kana/Abecedarium Latinum)
保川 清/ヤスカワ キヨシ/Kiyoshi Yasukawa
Primary department(Org1/Org2/Org3/Job title)
農学研究科/食品生物科学専攻食品生命科学講座/教授
Faculty
Org1 Org2 Org3 Job title
農学部
Contact address
Type Address(Japanese) Address(English)
Office 京都府京都市左京区北白川追分町 Kitashirakawa, Sakyo-ku, Kyoto, 6068502, Japan
Phone
Type Number
Office 075-753-6266
Affiliated academic organization(s) (Japanese)
Organization name(Japanese) Organization name(English)
日本生化学会 Japanese Biochemical Society
日本農芸化学会 Japan Society for Bioscience, Biotechnology, and Agrochemistry
日本生物工学会 The Society for Biotechnology, Japan
Academic degree
Field(Japanese) Field(English) University(Japanese) University(English) Method
理学修士 東京大学
医学博士 大阪大学
Graduate school
University(Japanese) University(English) Faculty(Japanese) Faculty(English) Major(Japanese) Major(English) Degree
東京大学 大学院理学系研究科修士課程生物化学専攻 修了
Work Experience
Period Organization(Japanese) Organization(English) Job title(Japanese) Job title(English)
1984/04/02-2004/09/30 東ソー株式会社 Tosoh Corporation
2004/10/01-2007/03/31 京都大学 Kyoto University 助教授 Associate Professor
2007/04/1-2013/07/31 京都大学 Kyoto University 准教授 Associate Professor
2013/08/01- 京都大学 Kyoto University 教授 Professor
Personal Profile
(Japanese)
酵素は生物の営む多様な反応を効率よく行うだけでなく、産業上でも広く応用されている。今後、エネルギーや環境という視点から酵素利用はますます重要視されるであろう。私は遺伝子工学、蛋白質工学を駆使して有用酵素を創製し、酵素の新しい応用面を切り開き、食品工業と医薬工業に酵素を利用することを目指している。さらに、酵素の構造と機能の関係を分子レベルで理解することにも取り組みたい。
(English)
Enzymes are proteins having catalytic activity. Biological reactions are accelerated and accurately controlled by enzymes in organisms. On the other hand, enzymes are widely used in industry. We are aiming at elucidating the relationship between structure and function of enzymes at a molecular level and developing new methods for application of enzymes in food and medical technologies by generating new enzyme functions and controlling enzyme reactions. We are focusing our activities mainly on the following subjects.
Research Topics
(Japanese)
酵素の機能改変と用途拡大
(English)
Generation and application of enzyme functions
Overview of your research
(Japanese)
酵素の構造と機能の関係を分子レベルで理解することと、有用酵素の創製や酵素反応の制御を通して酵素の新しい応用面を切り開き、食品工業と医薬工業への酵素利用を目指している。
(English)
We are aiming at elucidating the relationship between structure and function of enzymes at a molecular level and developing new methods for application of enzymes in food and medical technologies by generating new enzyme functions and controlling enzyme reactions.
Fields of research (key words)
Key words(Japanese) Key words(English)
酵素 Enzyme
バイオテクノロジー Biotechnology
触媒活性 Catalytic acitivty
安定性 Stability
Published Papers > Published Papers
(文系研究者の一般的な論文集への寄稿や単行本の分担執筆などは「著書等」を参照してください)
Authors Title Bibliography Date Peer-Review Language
Samukange, V., Yasukawa, K., and Inouye, K. Effects of heparin and cholesterol sulfate on the activity and stability of human matrix metalloproteinase 7 Biosci. Biotechnol. Biochem.,78,41-48 2014 Refereed
Konishi, A., Ma, X., and Yasukawa, K. Stabilization of Moloney murine leukemia virus reverse transcriptase by site-directed mutagenesis of the surface residue Val433 Biosci. Biotechnol. Biochem. 78: 147-150,78,147-150 2014 Refereed English
Samukange, V., Kamo, M., Yasukawa, K., and Inouye, K. Effects of salts on the Interaction of 8-anilinonaphthalene 1-sulphonate and thermolysin Biosci. Biotechnol. Biochem.,78,522-528 2014 Refereed English
Hisayoshi, T., Shinomura, M., Konishi, A., Tanaka, J., Shimoda, H., Hata, K., Takahashi, S., and Yasukawa, K. Inhibition of HIV-1 reverse transcriptase activity by Brasenia schreberi (Junsai) components J. Biol. Macromol.,14,59-65 2014 Refereed English
Konishi, A., Hisayoshi, T. Yokokawa, K., Barrioluengo, V., Menéndez-Arias, L., and Yasukawa, K. Amino acid substitutions away from the RNase H catalytic site increase the thermal stability of Moloney murine leukemia virus reverse transcriptase through RNase H inactivation Biophys. Res. Commun.,454,269-274 2014 Refereed English
Y. Kawasaki; K. Yasukawa; K. Inouye Effects of site-directed mutagenesis in the N-terminal domain of thermolysin on its stabilization Journal of Biochemistry,153,1,85-92 2013/01 Refereed English
A. Konishi; M. Shinomura; K. Yasukawa Enzymatic characterization of human immunodeficiency virus type 1 reverse transcriptase for use in cDNA synthesis Applied Biochemistry and Biotechnology,169,1,77-87 2013/01 Refereed English
E. Menach; K. Yasukawa; K. Inouye Effects of mutations of thermolysin, Asn116 to Asp and Asp150 to Glu, on salt-induced activation and stabilization Bioscience, Biotechnology and Biochemistry,77,4,741-746 2013 Refereed English
E. Menach; Y. Hashida; K. Yasukawa; K. Inouye Effects of conversion of the zinc-binding motif sequence of thermolysin, HEXXH, to that of dipeptidyl peptidase III, HEXXXH, on the activity and stability of thermolysin Bioscience, Biotechnology and Biochemistry,77,9,1901-1906 2013 Refereed English
K. Nishimura; M. Shinomura; A. Konishi; K. Yasukawa Stabilization of human immunodeficiency virus type 1 reverse transcriptase by site-directed mutagenesis Biotechnology Letters,35,12,2165-2175 2013 Refereed English
K. Yasukawa; A. Konishi; M. Shinomura; E. Nagaoka; S. Fujiwara Kinetic analysis of reverse transcriptase activity of bacterial family A DNA polymerases Biochemical and Biophysical Research Communications,427,3,654-658 2012/10/26 Refereed English
E. Menach; K. Yasukawa; K. Inouye Effects of site-directed mutagenesis of Asn116 in the β-hairpin of the N-terminal domain of thermolysin on its activity and stability Journal of Biochemistry,152,3,231-239 2012/09 Refereed English
A. Konishi; K. Yasukawa; K. Inouye Improving the thermal stability of avian myeloblastosis virus reverse transcriptase α-subunit by site-directed mutagenesis Biotechnology Letters,34,7,1209-1215 2012/07 Refereed English
V. Samukange; K. Yasukawa; K. Inouye Interaction of 8-anilinonaphthalene 1-sulphonate (ANS) and human matrix metalloproteinase 7 (MMP-7) as examined by MMP-7 activity and ANS fluorescence Journal of Biochemistry,151,5,533-540 2012/05 Refereed English
S. Sekiguchi; Y. Hashida; K. Yasukawa; K. Inouyey Stabilization of bovine intestine alkaline phosphatase by sugars Bioscience, Biotechnology and Biochemistry,76,1,95-100 2012/01 Refereed English
A. Morishima; K. Yasukawa; K. Inouye A possibility of a protein-bound water molecule as the ionizable group responsible for pKe at the alkaline side in human matrix metalloproteinase 7 activity Journal of Biochemistry,151,5,501-509 2012 Refereed English
H. Takeharu; K. Yasukawa; K. Inouye Thermodynamic analysis of ionizable groups involved in the catalytic mechanism of human matrix metalloproteinase 7 (MMP-7) Biochimica et Biophysica Acta - Proteins and Proteomics,1814,12,1940-1946 2011/12 Refereed English
S. Sekiguchi; K. Yasukawa; K. Inouye Effects of polyethylene glycol on bovine intestine alkaline phosphatase activity and stability Bioscience, Biotechnology and Biochemistry,75,11,2132-2137 2011/11 Refereed English
T. Miyake; K. Yasukawa; K. Inouye Analysis of the mechanism of inhibition of human matrix metalloproteinase 7 (MMP-7) activity by green tea catechins Bioscience, Biotechnology and Biochemistry,75,8,1564-1569 2011/08 Refereed English
A. Konishi; D. Nemoto; K. Yasukawa; K. Inouye Comparison of the thermal stabilities of the βα heterodimer and the α subunit of avian myeloblastosis virus reverse transcriptase Bioscience, Biotechnology and Biochemistry,75,8,1618-1620 2011/08 Refereed English
S. Sekiguchi; Y. Hashida; K. Yasukawa; K. Inouye Effects of amines and aminoalcohols on bovine intestine alkaline phosphatase activity Enzyme and Microbial Technology,49,2,171-176 2011/07/10 Refereed English
S. Sekiguchi; H. Kohno; K. Yasukawa; K. Inouye Chemiluminescent enzyme immunoassay for measuring leptin Bioscience, Biotechnology and Biochemistry,75,4,752-756 2011/04 Refereed English
E. Menach; K. Yasukawa; K. Inouye Effects of site-directed mutagenesis of the loop residue of the N-terminal domain Gly117 of thermolysin on its catalytic activity Bioscience, Biotechnology and Biochemistry,74,12,2457-2462 2010/12 Refereed English
K. Yasukawa; M. Mizuno; A. Konishi; K. Inouye Increase in thermal stability of Moloney murine leukaemia virus reverse transcriptase by site-directed mutagenesis Journal of Biotechnology,150,3,299-306 2010/11 Refereed English
K. Yasukawa; A. Konishi; K. Inouye Effects of organic solvents on the reverse transcription reaction catalyzed by reverse transcriptases from avian myeloblastosis virus and moloney murine leukemia virus Bioscience, Biotechnology and Biochemistry,74,9,1925-1930 2010/09 Refereed English
M. Kusano; K. Yasukawa; K. Inouye Effects of the mutational combinations on the activity and stability of thermolysin Journal of Biotechnology,147,1,7-16 2010/05/03 Refereed English
K. Yasukawa; N. Agata; K. Inouye Detection of cesA mRNA from Bacillus cereus by RNA-specific amplification Enzyme and Microbial Technology,46,5,391-396 2010/04/05 Refereed English
M. Kusano; K. Yasukawa; K. Inouye Synthesis of N-carbobenzoxy-l-aspartyl-l-phenylalanine methyl ester catalyzed by thermolysin variants with improved activity Enzyme and Microbial Technology,46,3-4,320-325 2010/03/05 Refereed English
M. Mizuno; K. Yasukawa; K. Inouye Insight into the mechanism of the stabilization of moloney murine leukaemia virus reverse transcriptase by eliminating RNase H activity Bioscience, Biotechnology and Biochemistry,74,2,440-442 2010/02 Refereed English
H. Sakurama; T. Takita; B. Mikami; T. Itoh; K. Yasukawa; K. Inouye Two crystal structures of lysyl-tRNA synthetase from Bacillus stearothermophilus in complex with lysyladenylate-like compounds: Insights into the irreversible formation of the enzyme-bound adenylate of L-lysine hydroxamate Journal of Biochemistry,145,5,555-563 2009/05 Refereed English
K. Asaoka; K. Yasukawa; K. Inouye Coagulation of soy proteins induced by thermolysin and comparison of the coagulation reaction with that induced by subtilisin Carlsberg Enzyme and Microbial Technology,44,4,229-234 2009/04/06 Refereed English
K. Yasukawa; M. Mizuno; K. Inouye Characterization of Moloney murine leukaemia virus/avian myeloblastosis virus chimeric reverse transcriptases Journal of Biochemistry,145,3,315-324 2009/03 Refereed English
K. Inouye; K. Nakano; K. Asaoka; K. Yasukawa Effects of thermal treatment on the coagulation of soy proteins induced by subtilisin Carlsberg Journal of Agricultural and Food Chemistry,57,2,717-723 2009/01/28 Refereed English
M. Kusano; K. Yasukawa; K. Inouye Insights into the catalytic roles of the polypeptide regions in the active site of thermolysin and generation of the thermolysin variants with high activity and stability Journal of Biochemistry,145,1,103-113 2009/01 Refereed English
K. Inouye; M. Nakano; K. Asaoka; K. Yasukawa Erratum: Effects of Thermal Treatment on the Coagulation of Soy Proteins Induced by Subtilisin Carlsberg (Journal of Agricultural and Food Chemistry 57 (717)) Journal of Agricultural and Food Chemistry,57,24,11581- 2009 Refereed English
T. Takita; T. Aono; H. Sakurama; T. Itoh; T. Wada; M. Minoda; K. Yasukawa; K. Inouye Effects of introducing negative charges into the molecular surface of thermolysin by site-directed mutagenesis on its activity and stability Biochimica et Biophysica Acta - Proteins and Proteomics,1784,3,481-488 2008/03 Refereed English
K. Yasukawa; D. Nemoto; K. Inouye Comparison of the thermal stabilities of reverse transcriptases from avian myeloblastosis virus and moloney murine leukaemia virus Journal of Biochemistry,143,2,261-268 2008/02 Refereed English
K. Yasukawa; K. Inouye Improving the activity and stability of thermolysin by site-directed mutagenesis Biochimica et Biophysica Acta - Proteins and Proteomics,1774,10,1281-1288 2007/10 Refereed English
K. Inouye; T. Shimada; K. Yasukawa Effects of neutral salts and alcohols on the activity of Streptomyces caespitosus neutral protease Journal of Biochemistry,142,3,317-324 2007/09 Refereed English
K. Inouye; K. Nakamura; M. Kusano; K. Yasukawa Improvement in performance of affinity gels containing Gly-D-Phe as a ligand to thermolysin due to increasing the spacer chain length Bioscience, Biotechnology and Biochemistry,71,8,2083-2086 2007/08 Refereed English
K. Yasukawa; M. Kusano; K. Inouye A new method for the extracellular production of recombinant thermolysin by co-expressing the mature sequence and pro-sequence in Escherichia coli Protein Engineering, Design and Selection,20,8,375-383 2007/08 Refereed English
K. Inouye; T. Shimada; K. Yasukawa Purification to homogeneity of a neutral metalloproteinase from Streptomyces caespitosus Bioscience, Biotechnology and Biochemistry,71,7,1773-1776 2007/07 Refereed English
C. Tatsumi; Y. Hashida; K. Yasukawa; K. Inouye Effects of site-directed mutagenesis of the surface residues Gln128 and Gln225 of thermolysin on its catalytic activity Journal of Biochemistry,141,6,835-842 2007/06 Refereed English
K. Inouye; M. Kusano; Y. Hashida; M. Minoda; K. Yasukawa Engineering, expression, purification, and production of recombinant thermolysin Biotechnology Annual Review,13,43-64 2007 Refereed English
M. Kusano; K. Yasukawa; Y. Hashida; K. Inouye Engineering of the pH-dependence of thermolysin activity as examined by site-directed mutagenesis of Asn112 located at the active site of thermolysin Journal of Biochemistry,139,6,1017-1023 2006/06 Refereed English
K. Inouye; M. Minoda; T. Takita; H. Sakurama; Y. Hashida; M. Kusano; K. Yasukawa Extracellular production of recombinant thermolysin expressed in Escherichia coli, and its purification and enzymatic characterization Protein Expression and Purification,46,2,248-255 2006/04 Refereed English
K. Yasukawa; M. Kusano; K. Nakamura; K. Inouye Characterization of Gly-D-Phe, Gly-L-Leu, and D-Phe as affinity ligands to thermolysin Protein Expression and Purification,46,2,332-336 2006/04 Refereed English
T. Tatekawa; H. Ogawa; M. Kawakami; Y. Oka; K. Yasukawa; H. Sugiyama; I. Kawase; T. Soma A novel direct competitive repopulation assay for human hematopoietic stem cells using NOD/SCID mice Cytotherapy,8,4,390-398 2006 Refereed English
S. Takakura; S. Tsuchiya; Y. Isawa; K. Yasukawa; T. Hayashi; M. Tomita; K. Suzuki; T. Hasegawa; T. Tagami; A. Kurashima; S. Ichiyama Rapid detection of Mycobacterium tuberculosis in respiratory samples by transcription-reverse transcription concerted reaction with an automated system Journal of Clinical Microbiology,43,11,5435-5439 2005/11 Refereed English
S. Takakura; S. Tsuchiya; N. Fujihara; T. Kudo; Y. Iinuma; S. Mitarai; S. Ichiyama; K. Yasukawa; T. Ishiguro Isothermal RNA sequence amplification method for rapid antituberculosis drug susceptibility testing of Mycobacterium tuberculosis Journal of Clinical Microbiology,43,5,2489-2491 2005/05 Refereed English
N. Masuda; K. Yasukawa; Y. Isawa; R. Horie; J. Saitoh; T. Ishiguro; Y. Nakaguchi; M. Nishibuchi; T. Hayashi Rapid detection of tdh and trh mRNAs of Vibrio parahaemolyticus by the transcription-reverse transcription concerted (TRC) method Journal of Bioscience and Bioengineering,98,4,236-243 2004/10 Refereed English
Y. Nakaguchi; T. Ishizuka; S. Ohnaka; T. Hayashi; K. Yasukawa; T. Ishiguro; M. Nishibuchi Rapid and specific detection of tdh, trh1, and trh2 mRNA of Vibrio parahaemolyticus by transcription-reverse transcription concerted reaction with an automated system Journal of Clinical Microbiology,42,9,4284-4292 2004/09 Refereed English
K. Yasukawa; S. Tsuchiya; T. Ekida; H. Iida; T. Ide; D. Miki; H. Yagame; K. Murayama; T. Ishiguro Fusion protein of interleukin-6 and interleukin-6 receptor without a polypeptide linker Journal of Bioscience and Bioengineering,96,1,38-46 2003/07 Refereed English
T. Ishiguro; J. Saitoh; R. Horie; T. Hayashi; T. Ishizuka; S. Tsuchiya; K. Yasukawa; T. Kido; Y. Nakaguchi; M. Nishibuchi; K. Ueda Intercalation activating fluorescence DNA probe and its application to homogeneous quantification of a target sequence by isothermal sequence amplification in a closed vessel Analytical Biochemistry,314,1,77-86 2003/03/01 Refereed English
T. Takizawa; M. Yanagisawa; W. Ochiai; K. Yasukawa; T. Ishiguro; K. Nakashima; T. Taga Directly linked soluble IL-6 receptor-IL-6 fusion protein induces astrocyte differentiation from neuroepithelial cells via activation of STAT3 Cytokine,13,5,272-279 2001/03/07 Refereed English
H. Mizuguchi; H. Mizuno; K. Yasukawa; T. Ishiguro; K. Fukui; T. Imanaka; M. Takagi Enhanced signal transduction by a directly fused protein of interleukin-6 and its receptor Journal of Bioscience and Bioengineering,91,3,299-304 2001/03 Refereed English
H. Minamiguchi; N. Yahata; T. Kimura; H. Fujiki; S. Harada; J. Wang; K. Okuda; H. Kaneko; K. Hodohara; T. Banba; K. Yasukawa; J.H. Ohyashiki; K. Ohyashiki; T. Abe; Y. Sonoda Interleukin 6 receptor expression by human cord blood- or peripheral blood-derived primitive haematopoietic progenitors implies acquisition of different functional properties British Journal of Haematology,110,2,327-338 2000/08 Refereed English
H. Mizuguchi; T. Kubomi; R. Nomura; K. Yasukawa; T. Imanaka; M. Takagi Screening of an oligopeptide antagonist for interleukin-6 from a random phage library Biotechnology Letters,22,12,1015-1020 2000/06 Refereed English
H. Mitani; N. Katayama; H. Araki; K. Ohishi; K. Kobayashi; H. Suzuki; K. Nishii; M. Masuya; K. Yasukawa; N. Minami; H. Shiku Activity of interleukin 6 in the differentiation of monocytes to macrophages and dendritic cells British Journal of Haematology,109,2,288-295 2000/05 Refereed English
T. Ueda; K. Tsuji; H. Yoshino; Y. Ebihara; H. Yagasaki; H. Hisakawa; T. Mitsui; A. Manabe; R. Tanaka; K. Kobayashi; M. Ito; K. Yasukawa; T. Nakahata Expansion of human NOD/SCID-repopulating cells by stem cell factor, Flk2/Flt3 ligand, thrombopoietin, IL-6, and soluble IL-6 receptor Journal of Clinical Investigation,105,7,1013-1021 2000/04 Refereed English
T. Kimura; J. Wang; H. Minamiguchi; H. Fujiki; S. Harada; K. Okuda; H. Kaneko; S. Yokota; K. Yasukawa; T. Abe; Y. Sonoda Signal through gp130 activated by soluble interleukin (IL)-6 receptor (R) and IL-6 or IL-6R/IL-6 fusion protein enhances ex vivo expansion of human peripheral blood-derived hematopoietic progenitors Stem Cells,18,6,444-452 2000 Refereed English
X. Sui; K. Tsuji; Y. Ebihara; R. Tanaka; K. Muraoka; M. Yoshida; K. Yamada; K. Yasukawa; T. Taga; T. Kishimoto; T. Nakahata Soluble interleukin-6 (IL-6) receptor with IL-6 stimulates megakaryopoiesis from human CD34<sup>+</sup> cells through glycoprotein (gp)130 signaling Blood,93,8,2525-2532 1999/04/15 Refereed English
T. Hada; M. Kondo; K. Yasukawa; Y. Amuro; K. Higashino Discrimination of liver cirrhosis from chronic hepatitis by measuring the ratio of Aleuria aurantia lectin-reactive serum cholinesterase to immunoreactive protein Clinica Chimica Acta,281,1-2,37-46 1999/03 Refereed English
R.L. Moritz; L.D. Ward; G.-F. Tu; L.J. Fabri; H. Ji; K. Yasukawa; R.J. Simpson The N-terminus of gp130 is critical for the formation of the high-affinity interleukin-6 receptor complex Growth Factors,16,4,265-278 1999 Refereed English
C. Song; G. Kenis; A. Van Gastel; E. Bosmans; A. Lin; R. De Jong; H. Neels; S. Scharpé; A. Janca; K. Yasukawa; M. Maes Influence of psychological stress on immune-inflammatory variables in normal humans. Part II. Altered serum concentrations of natural anti- inflammatory agents and soluble membrane antigens of monocytes and T lymphocytes Psychiatry Research,85,3,293-303 1999 Refereed English
J.-G. Zhang; Y. Zhang; C.M. Owczarek; L.D. Ward; R.L. Moritz; R.J. Simpson; K. Yasukawa; N.A. Nicola Identification and characterization of two distinct truncated forms of gp130 and a soluble form of leukemia inhibitory factor receptor α-chain in normal human urine and plasma Journal of Biological Chemistry,273,17,10798-10805 1998/04/24 Refereed English
Nishida, S; Okimoto, N; Okazaki, Y; Yamaguchi, A; Kumegawa, M; Yasukawa, K; Murayama, K; Nakamura, T Effect of monoclonal anti-human GP130 antibody (GPX7) on bone turnover in normal and ovariectomized rats CALCIFIED TISSUE INTERNATIONAL,62,3,227-236 1998/03 Refereed English
R. Nishimura; K. Moriyama; K. Yasukawa; G.R. Mundy; T. Yoneda Combination of interleukin-6 and soluble interleukin-6 receptors induces differentiation and activation of JAK-STAT and MAP kinase pathways in MG-63 human osteoblastic cells Journal of Bone and Mineral Research,13,5,777-785 1998 Refereed English
T. Kimura; H. Sakabe; S. Tanimukai; T. Abe; Y. Urata; K. Yasukawa; A. Okano; T. Taga; H. Sugiyama; T. Kishimoto; Y. Sonoda Simultaneous activation of signals through gp130, c-kit, and interleukin-3 receptor promotes a trilineage blood cell production in the absence of terminally acting lineage-specific factors Blood,90,12,4767-4778 1997/12/15 Refereed English
M. Okamoto; K. Yasukawa; K. Katsuura; M. Baba Inhibition of interleukin-6-induced human immunodeficiency virus type 1 expression by anti-gp130 monoclonal antibody Biochemistry and Molecular Biology International,43,4,733-740 1997/11 Refereed English
T. Matsuba; M. Yano; N. Abiru; H. Takino; S. Akazawa; S. Nagataki; K. Yasukawa Expression of recombinant human glutamic acid decarboxylase (GAD) in myeloma cells and enzyme-linked immunosorbent assay (ELISA) for autoantibodies to GAD Journal of Biochemistry,121,1,20-24 1997/01 Refereed English
Y. Nakajima; O. Mikami; M. Yoshioka; Y. Motoi; T. Ito; Y. Ishikawa; M. Fuse; K. Nakano; K. Yasukawa Elevated levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) activities in the sera and milk of cows with naturally occurring coliform mastitis Research in Veterinary Science,62,3,297-298 1997 Refereed English
F.A. Montero-Julian; H. Brailly; C. Sautès; I. Joyeux; T. Dorval; V. Mosseri; K. Yasukawa; J. Wijdenes; A. Adler; I. Gorin; W.H. Fridman; E. Tartour Characterization of soluble gp130 released by melanoma cell lines: A polyvalent antagonist of cytokines from the interleukin 6 family Clinical Cancer Research,3,8,1443-1451 1997 Refereed English
P. Vollmer; M. Peters; M. Ehlers; H. Yagame; T. Matsuba; M. Kondo; K. Yasukawa; K.-H. Meyer Zum Büschenfelde; S. Rose-John Yeast expression of the cytokine receptor domain of the soluble interleukin-6 receptor Journal of Immunological Methods,199,1,47-54 1996/11/29 Refereed English
T. Taya; K. Yasukawa; K. Miyata; S.-I. Kidokoro; K. Inouye Genetically engineered antibodies which have reduced binding affinity for thyroxine but retain the same affinity for tri-iodothyronine Biotechnology Letters,18,11,1325-1330 1996/11 Refereed English
S. Tajima; K. Tsuji; Y. Ebihara; X. Sui; R. Tanaka; K. Muraoka; M. Yoshida; K. Yamada; K. Yasukawa; T. Taga; T. Kishimoto; T. Nakahata Analysis on interleukin 6 receptor and gp130 expressions and proliferative capability of human CD34<sup>+</sup> cells Journal of Experimental Medicine,184,4,1357-1364 1996/10/01 Refereed English
T. Azuma; M. Hirai; S. Ito; K. Yamamoto; R.T. Taggart; T. Matsuba; K. Yasukawa; K. Uno; T. Hayakumo; M. Nakajima Expression of cathepsin E in pancreas: A possible tumor marker for pancreas, a preliminary report International Journal of Cancer,67,4,492-497 1996/08/07 Refereed English
M. Kondo; T. Taya; T. Matsuba; N. Fushimi; K. Inouye; I. Kidokoro S.-; K. Yasukawa Use of a surface plasmon resonance biosensor for the determination of binding constants of transiently expressed recombinant antibody to its antigen Biotechnology Techniques,10,8,547-552 1996/08 Refereed English
K. Ikeda; M. Kinoshita; N. Tagaya; T. Shiojima; T. Taga; K. Yasukawa; H. Suzuki; A. Okano Coadministration of interleukin-6 (IL-6) and soluble IL-6 receptor delays progression of wobbler mouse motor neuron disease Brain Research,726,1-2,91-97 1996/07/08 Refereed English
M. Diamant; M.B. Hansen; K. Rieneck; M. Svenson; K. Yasukawa; K. Bendtzen Differential interleukin-6 (IL-6) responses of three established myeloma cell lines in the presence of soluble human IL-6 receptors Leukemia Research,20,4,291-301 1996/04 Refereed English
X. Sui; K. Tsuji; S. Tajima; R. Tanaka; K. Muraoka; Y. Ebihara; K. Ikebuchi; K. Yasukawa; T. Taga; T. Kishimoto; T. Nakahata Erythropoietin-independent erythrocyte production: Signals through gp130 and c-kit dramatically promote erythropoiesis from human CD34<sup>+</sup> cells Journal of Experimental Medicine,183,3,837-845 1996/03/01 Refereed English
M. Kondo; T. Hada; K. Fukui; A. Iwasaki; K. Higashino; K. Yasukawa Enzyme-linked immunosorbent assay (ELISA) for Aleuria aurantia lectin-reactive serum cholinesterase to differentiate liver cirrhosis and chronic hepatitis Clinica Chimica Acta,243,1,1-9 1995/12/15 Refereed English
YANG, ZM; LE, SP; CHEN, DB; COTA, J; SIERO, V; YASUKAWA, K; HARPER, MJK LEUKEMIA INHIBITORY FACTOR, LIF RECEPTOR, AND GP130 IN THE MOUSE UTERUS DURING EARLY-PREGNANCY MOLECULAR REPRODUCTION AND DEVELOPMENT,42,4,407-414 1995/12 Refereed English
T. Matsuba; M. Yamada; H. Suzuki; A. Kanai; O. Isozaki; T. Yoshida; T. Tsushima; K. Yasukawa Expression of recombinant human thyrotropin receptor in myeloma cells Journal of Biochemistry,118,2,265-270 1995/08 Refereed English
F.D. De Hon; E. Ten Boekel; J. Herrman; C. Clement; M. Ehlers; T. Taga; K. Yasukawa; Y. Ohsugi; T. Kishimoto; S. Rose-John; J. Wijdenes; R. Kastelein; L.A. Aarden; J.P.J. Brakenhoff Functional distinction of two regions of human interleukin 6 important for signal transduction via gp130 Cytokine,7,5,398-407 1995/07 Refereed English
D. Guschin; N. Rogers; J. Briscoe; B. Witthuhn; D. Watling; F. Horn; S. Pellegrini; K. Yasukawa; P. Heinrich; G.R. Stark; J.N. Ihle; I.M. Kerr A major role for the protein tyrosine kinase JAK1 in the JAK/STAT signal transduction pathway in response to interleukin-6 EMBO Journal,14,7,1421-1429 1995/04/03 Refereed English
L.D. Ward; G.J. Hewlett; A. Hammacher; J. Weinstock; K. Yasukawa; R.J. Simpson; D.J. Winzor Use of a biosensor with surface plasmon resonance detection for the determination of binding constants: Measurement of interleukin-6 binding to the soluble interleukin-6 receptor Biochemistry,34,9,2901-2907 1995/03/07 Refereed English
Y. Iba; T. Kaneko; T. Ekida; K. Miyata; K. Inouye; Y. Kurosawa; K. Yasukawa A new system for the expression of recombinant antibody in mammalian cells Biotechnology Letters,17,2,135-138 1995/02 Refereed English
M.B. Hansen; M. Svenson; K. Abell; K. Yasukawa; M. Diamant; K. Bendtzen Influence of interleukin-6 (IL-6) autoantibodies on IL-6 binding to cellular receptors European Journal of Immunology,25,2,348-354 1995/02 Refereed English
K. Takagi; K. Yasukawa; Y. Iba; Y. Isobe; Y. Suketa; R. Kannagi; K. Kohda; M. Takagi; T. Imanaka Expression of recombinant antibody against cancer-specific carbohydrate Journal of Fermentation and Bioengineering,79,5,405-409 1995 Refereed English
L.D. Ward; G.J. Howlett; R.L. Moritz; A. Hammacher; K. Yasukawa; R.J. Simpson Studies of cytokine-cytokine receptor interactions: Influence of ligand dimerization Techniques in Protein Chemistry,6,C,417-425 1995 Refereed English
A. Hammacher; L.D. Ward; J. Weinstock; H. Treutlein; K. Yasukawa; R.J. Simpson Structure-function analysis of human IL-6: Identification of two distinct regions that are important for receptor binding Protein Science,3,12,2280-2293 1994/12 Refereed English
L.D. Ward; G.J. Howlett; G. Discolo; K. Yasukawa; A. Hammacher; R.L. Moritz; R.J. Simpson High affinity interleukin-6 receptor is a hexameric complex consisting of two molecules each of interleukin-6, interleukin-6 receptor, and gp-130 Journal of Biological Chemistry,269,37,23286-23289 1994/09/16 Refereed English
M. Diamant; M.B. Hansen; K. Rieneck; M. Svenson; K. Yasukawa; K. Bendtzen Stimulation of the B9 hybridoma cell line by soluble interleukin-6 receptors Journal of Immunological Methods,173,2,229-235 1994/08/01 Refereed English
E. Dittrich; S. Rose-John; C. Gerhartz; J. Müllberg; T. Stoyan; K. Yasukawa; P.C. Heinrich; L. Graeve Identification of a region within the cytoplasmic domain of the interleukin-6 (IL-6) signal transducer gp130 important for ligand-induced endocytosis of the IL-6 receptor Journal of Biological Chemistry,269,29,19014-19020 1994/07/22 Refereed English
Z.-Y. Lu; X.-G. Zhang; Z.-J. Gu; K. Yasukawa; M. Amiot; M. Etrilland; R. Bataille; B. Klein A highly sensitive quantitative bioassay for human interleukin-11 Journal of Immunological Methods,173,1,19-26 1994/07/12 Refereed English
C. Gerhartz; E. Dittrich; T. Stoyan; S. Rose-John; K. Yasukawa; P.C. Heinrich; L. Graeve Biosynthesis and half-life of the interleukin-6 receptor and its signal transducer gp130 European Journal of Biochemistry,223,1,265-274 1994/07/01 Refereed English
YIN, TG; YASUKAWA, K; TAGA, T; KISHIMOTO, T; YANG, YC IDENTIFICATION OF A 130-KILODALTON TYROSINE-PHOSPHORYLATED PROTEIN-INDUCED BY INTERLEUKIN-11 AS JAK2 TYROSINE KINASE, WHICH ASSOCIATES WITH GP130 SIGNAL TRANSDUCER EXPERIMENTAL HEMATOLOGY,22,5,467-472 1994/05 Refereed English
X.-G. Zhang; J.-J. Gu; Z.-Y. Lu; K. Yasukawa; G.D. Yancopoulos; K. Turner; M. Shoyab; T. Taga; T. Kishimoto; R. Bataille; B. Klein Ciliary neurotropic factor, interleukin 11, leukemia inhibitory factor, and oncostatin M are growth factors for human myeloma cell lines using the interleukin 6 signal transducer GP130 Journal of Experimental Medicine,179,4,1337-1342 1994/04/01 Refereed English
TAKAGI, K; ISOBE, Y; YASUKAWA, K; OKOUCHI, E; SUKETA, Y NITRIC-OXIDE BLOCKS THE CELL-CYCLE OF MOUSE MACROPHAGE-LIKE CELLS IN THE EARLY G(2)+M PHASE FEBS LETTERS,340,3,159-162 1994/03/07 Refereed English
K. Yoshida; I. Chambers; J. Nichols; A. Smith; M. Saito; K. Yasukawa; M. Shoyab; T. Taga; T. Kishimoto Maintenance of the pluripotential phenotype of embryonic stem cells through direct activation of gp130 signalling pathways Mechanisms of Development,45,2,163-171 1994/02 Refereed English
C. Lutticken; U.M. Wegenka; J. Yuan; J. Buschmann; C. Schindler; A. Ziemiecki; A.G. Harpur; A.F. Wilks; K. Yasukawa; T. Taga; T. Kishimoto; G. Barbieri; S. Pellegrini; M. Sendtner; P.C. Heinrich; F. Horn Association of transcription factor APRF and protein kinase Jak1 with the interleukin-6 signal transducer gp130 Science,263,5143,89-92 1994/01/07 Refereed English
YIN, TG; TAGA, T; TSANG, MLS; YASUKAWA, K; KISHIMOTO, T; YANG, YC INVOLVEMENT OF IL-6 SIGNAL TRANSDUCER GP130 IN IL-11-MEDIATED SIGNAL-TRANSDUCTION JOURNAL OF IMMUNOLOGY,151,5,2555-2561 1993/09/01 Refereed English
L.D. Ward; A. Hammacher; J.-G. Zhang; J. Weinstock; K. Yasukawa; C.J. Morton; R.S. Norton; R.J. Simpson Role of the C-terminus in the activity, conformation, and stability of interleukin-6 Protein Science,2,9,1472-1481 1993/09 Refereed English
M. Narazaki; K. Yasukawa; T. Saito; Y. Ohsugi; H. Fukui; Y. Koishihara; G.D. Yancopoulos; T. Taga; T. Kishimoto Soluble forms of the interleukin-6 signal-transducing receptor component gp130 in human serum possessing a potential to inhibit signals through membrane-anchored gp130 Blood,82,4,1120-1126 1993/08/15 Refereed English
SAITO, T; TAGA, T; MIKI, D; FUTATSUGI, K; YAWATA, H; KISHIMOTO, T; YASUKAWA, K PREPARATION OF MONOCLONAL-ANTIBODIES AGAINST THE IL-6 SIGNAL TRANSDUCER, GP130, THAT CAN INHIBIT IL-6-MEDIATED FUNCTIONS JOURNAL OF IMMUNOLOGICAL METHODS,163,2,217-223 1993/08/09 Refereed English
M. Murakami; M. Hibi; N. Nakagawa; T. Nakagawa; K. Yasukawa; K. Yamanishi; T. Taga; T. Kishimoto IL-6-induced homodimerization of gp130 and associated activation of a tyrosine kinase Science,260,5115,1808-1810 1993/06/18 Refereed English
H. Yawata; K. Yasukawa; S. Natsuka; M. Murakami; K. Yamasaki; M. Hibi; T. Taga; T. Kishimoto Structure - Function analysis of human IL-6 receptor: Dissociation of amino acid residues required for IL-6-binding and for IL-6 signal transduction through gp130 EMBO Journal,12,4,1705-1712 1993/04 Refereed English
J.-P. Gaillard; R. Bataille; H. Brailly; C. Zuber; K. Yasukawa; M. Attal; N. Maruo; T. Taga; T. Kishimoto; B. Klein Increased and highly stable levels of functional soluble interleukin-6 receptor in sera of patients with monoclonal gammopathy European Journal of Immunology,23,4,820-824 1993/04 Refereed English
Y. Nakajima; E. Momotani; T. Murakami; Y. Ishikawa; M. Morimatsu; M. Saito; H. Suzuki; K. Yasukawa Induction of acute phase protein by recombinant human interleukin-6 (IL-6) in calves Veterinary Immunology and Immunopathology,35,3-4,385-391 1993/01 Refereed English
T. Kaneko; Y. Iba; K. Zenita; K. Shigeta; K. Nakano; W. Itoh; Y. Kurosawa; R. Kannagi; K. Yasukawa Preparation of mouse-human chimeric antibody to an embryonic carbohydrate antigen, Lewis Y Journal of Biochemistry,113,1,114-117 1993/01 Refereed English
H. Suzuki; K. Yasukawa; T. Saito; M. Narazaki; A. Hasegawa; T. Taga; T. Kishimoto Serum soluble interleukin-6 receptor in MRL/lpr mice is elevated with age and mediates the interleukin-6 signal European Journal of Immunology,23,5,1078-1082 1993 Refereed English
J. Müllberg; E. Dittrich; L. Graeve; C. Gerhartz; K. Yasukawa; T. Taga; T. Kishimoto; P.C. Heinrich; S. Rose-John Differential shedding of the two subunits of the interleukin-6 receptor FEBS Letters,332,1-2,174-178 1993 Refereed English
I. Kobayashi; T. Matsuda; T. Saito; K. Yasukawa; H. Kikutani; T. Hirano; T. Taga; T. Kishimoto Abnormal distribution of IL-6 receptor in aged MRL/lpr mice: Elevated expression on B cells and absence on CD4<sup>+</sup> cells International Immunology,4,12,1407-1412 1992/12 Refereed English
M. Yamaguchi; M. Michishita; K. Hirayoshi; K. Yasukawa; M. Okuma; K. Nagata Down-regulation of interleukin 6 receptors of mouse myelomonocytic leukemic cells by leukemia inhibitory factor Journal of Biological Chemistry,267,31,22035-22042 1992/11/05 Refereed English
NISHIMURA, C; EKIDA, T; NOMURA, K; SAKAMOTO, K; SUZUKI, H; YASUKAWA, K; KISHIMOTO, T; ARATA, Y ROLE OF LEUCINE RESIDUES IN THE C-TERMINAL REGION OF HUMAN INTERLEUKIN-6 IN THE BIOLOGICAL-ACTIVITY FEBS LETTERS,311,3,271-275 1992/10/26 Refereed English
J. Liu; B. Modrell; A. Aruffo; J.S. Marken; T. Taga; K. Yasukawa; M. Murakami; T. Kishimoto; M. Shoyab Interleukin-6 signal transducer gp130 mediates oncostatin M signaling Journal of Biological Chemistry,267,24,16763-16766 1992/08/25 Refereed English
H. Suzuki; K. Yasukawa; T. Saito; R. Goitsuka; A. Hasegawa; Y. Ohsugi; T. Taga; T. Kishimoto Anti-human interleukin-6 receptor antibody inhibits human myeloma growth in vivo European Journal of Immunology,22,8,1989-1993 1992/08 Refereed English
T. Saito; K. Futatsugi; D. Miki; H. Suzuki; K. Yasukawa An establishment of ELISA for murine IL-6 Biotechnology Techniques,6,4,365-370 1992/07 Refereed English
NOMOTO, H; YASUKAWA, K; INOUE, Y SEPARATION OF NEUTRAL GLYCOASPARAGINES ACCORDING TO THEIR CONTENT OF CIS DIOL GROUPS BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,56,7,1090-1095 1992/07 Refereed English
N.Y. Ip; S.H. Nye; T.G. Boulton; S. Davis; T. Taga; Y. Li; S.J. Birren; K. Yasukawa; T. Kishimoto; D.J. Anderson; N. Stahl; G.D. Yancopoulos CNTF and LIF Act on neuronal cells via shared signaling pathways that involve the IL-6 signal transducing receptor component gp130 Cell,69,7,1121-1132 1992/06/26 Refereed English
HONDA, M; YAMAMOTO, S; CHENG, M; YASUKAWA, K; SUZUKI, H; SAITO, T; OSUGI, Y; TOKUNAGA, T; KISHIMOTO, T HUMAN SOLUBLE IL-6 RECEPTOR - ITS DETECTION AND ENHANCED RELEASE BY HIV-INFECTION JOURNAL OF IMMUNOLOGY,148,7,2175-2180 1992/04/01 Refereed English
NAKAJIMA, T; YAMAMOTO, S; CHENG, M; YASUKAWA, K; HIRANO, T; KISHIMOTO, T; TOKUNAGA, T; HONDA, M SOLUBLE INTERLEUKIN-6 RECEPTOR IS RELEASED FROM RECEPTOR-BEARING CELL-LINES INVITRO JAPANESE JOURNAL OF CANCER RESEARCH,83,4,373-378 1992/04 Refereed English
K. Yasukawa; K. Futatsugi; T. Saito; H. Yawata; M. Narazaki; H. Suzuki; T. Taga; T. Kishimoto Association of recombinant soluble IL-6-signal transducer, gp130, with a complex of IL 6 and soluble IL-6 receptor, and establishment of an ELISA for soluble gp130 Immunology Letters,31,2,123-130 1992/02/01 Refereed English
T. Taga; M. Hibi; M. Murakami; M. Saito; H. Yawata; M. Narazaki; Y. Hirata; T. Sugita; K. Yasukawa; T. Hirano; T. Kishimoto Interleukin-6 receptor and signals Chemical Immunology,51,181-204 1992 Refereed English
MIHARA, M; KOISHIHARA, Y; FUKUI, H; YASUKAWA, K; OHSUGI, Y MURINE ANTI-HUMAN IL-6 MONOCLONAL-ANTIBODY PROLONGS THE HALF-LIFE IN CIRCULATING BLOOD AND THUS PROLONGS THE BIOACTIVITY OF HUMAN IL-6 IN MICE IMMUNOLOGY,74,1,55-59 1991/09 Refereed English
H. Suzuki; K. Yasukawa; T. Saito; M. Anzai; R. Goitsuka; A. Hasegawa; Y. Ohsugi; T. Taga; T. Kishimoto Anti-murine IL-6 receptor antibody inhibits IL-6 effects in vivo Immunology Letters,30,1,17-22 1991/09 Refereed English
T. Saito; K. Yasukawa; H. Suzuki; K. Futatsugi; T. Fukunaga; C. Yokomizo; Y. Koishihara; H. Fukui; Y. Ohsugi; H. Yawata; I. Kobayashi; T. Hirano; T. Taga; T. Kishimoto Preparation of soluble murine IL-6 receptor and anti-murine IL-6 receptor antibodies Journal of Immunology,147,1,168-173 1991/07/01 Refereed English
K. Hirayoshi; A. Tsuru; M. Yamashita; M. Tomida; Y. Yamamoto-Yamaguchi; K. Yasukawa; M. Hozumi; D.V. Goeddel; K. Nagata Both D factor/LIF and IL-6 inhibit the differentation of mouse teratocarcinoma F9 cells FEBS Letters,282,2,401-404 1991/05/06 Refereed English
NISHIMURA, C; FUTATSUGI, K; YASUKAWA, K; KISHIMOTO, T; ARATA, Y SITE-SPECIFIC MUTAGENESIS OF HUMAN INTERLEUKIN-6 AND ITS BIOLOGICAL-ACTIVITY FEBS LETTERS,281,1-2,167-169 1991/04/09 Refereed English
NISHIMURA, C; EKIDA, T; MASUDA, S; FUTATSUGI, K; ITOH, S; YASUKAWA, K; KISHIMOTO, T; ARATA, Y CHEMICAL MODIFICATION AND H-1-NMR STUDIES ON THE RECEPTOR-BINDING REGION OF HUMAN INTERLEUKIN-6 EUROPEAN JOURNAL OF BIOCHEMISTRY,196,2,377-384 1991/03/14 Refereed English
NISHIMURA, C; HANZAWA, H; ITOH, S; YASUKAWA, K; SHIMADA, I; KISHIMOTO, T; ARATA, Y PROTON NUCLEAR-MAGNETIC-RESONANCE STUDY OF HUMAN INTERLEUKIN-6 - CHEMICAL MODIFICATIONS AND PARTIAL SPECTRAL ASSIGNMENTS FOR THE AROMATIC RESIDUES BIOCHIMICA ET BIOPHYSICA ACTA,1041,3,243-249 1990/12/05 Refereed English
K. Yasukawa; T. Saito; T. Fukunaga; Y. Sekimori; Y. Koishihara; H. Fukui; Y. Ohsugi; T. Matsuda; H. Yawata; T. Hirano; T. Taga; T. Kishimoto Purification and characterization of soluble human IL-6 receptor expressed in CHO cells Journal of Biochemistry,108,4,673-676 1990/10 Refereed English
K. Yasukawa; T. Saito A new system for the expression of human interleukin-6 in Eschrichia coli Biotechnology Letters,12,6,419-424 1990/06 Refereed English
GOITSUKA, R; OHASHI, T; ONO, K; YASUKAWA, K; KOISHIBARA, Y; FUKUI, H; OHSUGI, Y; HASEGAWA, A IL-6 ACTIVITY IN FELINE INFECTIOUS PERITONITIS JOURNAL OF IMMUNOLOGY,144,7,2599-2603 1990/04/01 Refereed English
T. Taga; M. Hibi; Y. Hirata; K. Yamasaki; K. Yasukawa; T. Matsuda; T. Hirano; T. Kishimoto Interleukin-6 triggers the association of its receptor with a possible signal transducer, gp130 Cell,58,3,573-581 1989/08/11 Refereed English
T. Hirano; T. Taga; K. Yamasaki; T. Matsuda; K. Yasukawa; Y. Hirata; H. Yawata; O. Tanabe; S. Akira; T. Kishimoto Molecular cloning of the cDNAs for interleukin-6/B cell stimulatory factor 2 and its receptor Annals of the New York Academy of Sciences,557,167-180 1989 Refereed English
T. Taga; M. Hibi; Y. Hirata; H. Yawata; S. Natsuka; K. Yasukawa; T. Totsuka; K. Yamasaki; T. Hirano; T. Kishimoto Interleukin-6 receptor and a unique mechanism of its signal transduction Cold Spring Harbor Symposia on Quantitative Biology,54,2,713-722 1989 Refereed English
Y. Asagoe; K. Yasukawa; T. Saito; N. Maruo; K. Miyata; T. Kono; T. Miyake; T. Kato; H. Kakidani; M. Mitani Human B-cell stimulatory factor-2 expressed in Escherichia coli Bio/Technology,6,7,806-809 1988 Refereed English
N. Maruo; K. Miyata; K. Yasukawa; T. Fukunaga; M. Mitani The production of BSF-2/IL-6 by human glioblastoma International Journal of Immunopharmacology,10,SUPPL. 1,14- 1988 Refereed English
K. Yasukawa; T. Hirano; Y. Watanabe; K. Muratani; T. Matsuda; S. Nakai; T. Kishimoto Structure and expression of human B cell stimulatory factor-2 (BSF-2/IL-6) gene. EMBO Journal,6,10,2939-2945 1987/10 Refereed English
T. Hirano; K. Yasukawa; H. Harada; T. Taga; Y. Watanabe; T. Matsuda; S. Kashiwamura; K. Nakajima; K. Koyama; A. Iwamatsu Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin Nature,324,6092,73-76 1986/11/06 Refereed English
WATANABE, K; YASUKAWA, K; ISO, K GRAPHIC DISPLAY OF NUCLEIC-ACID STRUCTURE BY A MICROCOMPUTER NUCLEIC ACIDS RESEARCH,12,1,801-809 1984 Refereed English

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Conference Presentation > Conference Presentation
(文系研究者の一般的な学会報告の業績は「講演等」を参照してください)
Authors Title Bibliography Date Peer-Review Language
X. Sui; K. Tsuji; R. Tanaka; S. Tajima; K. Muraoka; Y. Ebihara; K. Ikebuchi; K. Yasukawa; T. Taga; T. Kishimoto; T. Nakahata gp130 and c-Kit signalings synergize for ex vivo expansion of human primitive hemopoietic progenitor cells Proceedings of the National Academy of Sciences of the United States of America,92,7,2859-2863 1995/03/28 Refereed English
M. Narazaki; B.A. Witthuhn; K. Yoshida; O. Silvennoinen; K. Yasukawa; J.N. Ihle; T. Kishimoto; T. Taga Activation of JAK2 kinase mediated by the interleukin 6 signal transducer gp130 Proceedings of the National Academy of Sciences of the United States of America,91,6,2285-2289 1994/03/15 Refereed English
T. Taga; M. Narazaki; K. Yasukawa; T. Saito; D. Miki; M. Hamaguchi; S. Davis; M. Shoyab; G.D. Yancopoulos; T. Kishimoto Functional inhibition of hematopoietic and neurotrophic cytokines by blocking the interleukin 6 signal transducer gp130 Proceedings of the National Academy of Sciences of the United States of America,89,22,10998-11001 1992/11/15 Refereed English
M. Murakami; M. Narazaki; M. Hibi; H. Yawata; K. Yasukawa; M. Hamaguchi; T. Taga; T. Kishimoto Critical cytoplasmic region of the interleukin 6 signal transducer gp130 is conserved in the cytokine receptor family Proceedings of the National Academy of Sciences of the United States of America,88,24,11349-11353 1991/12 Refereed English
T. Hirano; T. Taga; K. Yasukawa; K. Nakajima; N. Nakano; F. Takatsuki; M. Shimizu; A. Murashima; S. Tsunasawa; F. Sakiyama Human B-cell differentiation factor defined by an anti-peptide antibody and its possible role in autoantibody production Proceedings of the National Academy of Sciences of the United States of America,84,1,228-231 1987/01 Refereed English
Lectures > Invited Lectures
(文系研究者の一般的な学会報告はこの項に収録されております)
Title Conference name Organization name Date Language
タンパク質工学による逆転写酵素の耐熱化(シンポジウム:タンパク質工学による耐熱化酵素創製の新しい戦略) 2011年度日本農芸化学会大会 2011/03/28 Japanese
逆転写酵素の活性と熱安定性(シンポジウム:核酸に関わる酵素の化学) 2009年度日本農芸化学会大会 2009/03/29 Japanese
基質特異性を改変させたことにより自己活性化能を消失したプロテアーゼの大腸菌での生産(タンパク質発現手法の最適化-より良い機能性タンパク質を求めて) 2009年度日本農芸化学会大会 2009/03/28 Japanese
Characterization of Moloney murine leukemia virus/avian myeloblastosis virus chimeric reverse transcriptase RNase H 2012 2012/09/05 English
Publications > Books
Authors Title Publisher Date Language
井上國世、保川清 アスパルテームの合成 酵素利用技術大系(小宮山眞監修)p. 632-636、株式会社エヌ・ティー・エス、東京、2010 2010 Japanese
保川清  核酸増幅法  産業酵素の応用技術と最新動向(井上國世、監修)p. 194-202、シーエムシー出版、東京、2009 2009 Japanese
井上國世、橋田泰彦、草野正雪、保川清  サーモライシンの応用と高機能化  産業酵素の応用技術と最新動向(井上國世、監修)p. 58-68、シーエムシー出版、東京、2009 2009 Japanese
Awards
Title(Japanese) Title(English) Organization name(Japanese) Organization name(English) Date
日本生物工学会論文賞Excellent Paper Aword of the Society for Biotechnology, Japan 1996
External funds, competitive funds and Grants-in-Aid for Scientific Research(kaken)
Type Position Title(Japanese) Title(English) Period
基盤研究(C) Representative 組換えAMV逆転写酵素発現系の構築と蛋白質工学による生産性、活性、熱安定性の向上 2007/04/01-2009/03/31
基盤研究(C) Representative 高機能逆転写酵素を用いたcDNA合成法とRNA増幅法の性能評価と用途拡大 2009/04/01-2011/03/31
基盤研究(C) Representative 耐熱型逆転写酵素の耐熱化機構の解明 (平成27年度分) 2015/04/01-2016/03/31
基盤研究(C) Representative 耐熱型逆転写酵素の耐熱化機構の解明 (平成28年度分) 2016/04/01-2017/03/31
Teaching subject(s)
Name(Japanese) Name(English) Term Department Period
食品基礎生物学Ⅰ Introduction to the Molecular Cell Biology -
食品分子生物学 Moelcular Biology of Foods -
酵素化学 Enzyme Chemistry -
酵素化学・生化学実験及び実験法 Laboratory Course in Enzyme Chemistry and Biochemistry -
酵素の作用と応用 後期 農学部 2011/04-2012/03
食品生化学I 前期 農学部 2011/04-2012/03
食品生化学Ⅱ 後期 農学部 2011/04-2012/03
酵素化学 後期 農学部 2011/04-2012/03
酵素化学・生化学実験及び実験法 前期 農学部 2011/04-2012/03
酵素化学専攻実験 農学研究科 2011/04-2012/03
酵素化学専攻演習 農学研究科 2011/04-2012/03
酵素化学特論 農学研究科 2011/04-2012/03
酵素の作用と応用 Enzymes: Function and Application 後期 農学部 2012/04-2013/03
酵素化学 Enzyme Chemistry 後期 農学部 2012/04-2013/03
酵素化学実験及び実験法 Laboratory Course in Enzyme Chemistry 前期 農学部 2012/04-2013/03
酵素を用いた高感度分析 Uses of enzymes in sensitive analyses 前期 全学共通科目 2012/04-2013/03
食品生化学I Food Biochemistry I 前期 農学部 2012/04-2013/03
食品生化学II Food Biochemistry II 後期 農学部 2012/04-2013/03
酵素の作用と応用 Enzymes: Function and Application 後期 農学部 2013/04-2014/03
食品生化学I Food Biochemistry I 前期 農学部 2013/04-2014/03
食品生化学II Food Biochemistry II 後期 農学部 2013/04-2014/03
酵素化学 Enzyme Chemistry 後期 農学部 2013/04-2014/03
酵素化学実験及び実験法 Laboratory Course in Enzyme Chemistry 前期 農学部 2013/04-2014/03
酵素化学特論 Enzyme Chemistry (Advanced Course) 後期集中 農学研究科 2013/04-2014/03
酵素化学専攻演習 Enzyme Chemistry Seminar 通年 農学研究科 2013/04-2014/03
酵素化学専攻実験 Experimental Course of Enzyme Chemistry 通年 農学研究科 2013/04-2014/03
酵素を用いた高感度分析 Uses of enzymes in sensitive analyses 前期 全学共通科目 2013/04-2014/03
酵素の作用と応用 Enzymes: Function and Application 後期 農学部 2014/04-2015/03
食品生化学I Food Biochemistry I 前期 農学部 2014/04-2015/03
食品生化学II Food Biochemistry II 後期 農学部 2014/04-2015/03
酵素化学 Enzyme Chemistry 後期 農学部 2014/04-2015/03
酵素化学実験及び実験法 Laboratory Course in Enzyme Chemistry 前期 農学部 2014/04-2015/03
食品分析化学 Analytical Chemistry in Food Science 後期 農学部 2014/04-2015/03
酵素化学専攻演習 Enzyme Chemistry Seminar 通年 農学研究科 2014/04-2015/03
酵素化学専攻実験 Experimental Course of Enzyme Chemistry 通年 農学研究科 2014/04-2015/03
酵素の作用と応用 Enzymes: Function and Application 後期 農学部 2015/04-2016/03
酵素化学 Enzyme Chemistry 後期 農学部 2015/04-2016/03
酵素化学実験及び実験法 Laboratory Course in Enzyme Chemistry 前期 農学部 2015/04-2016/03
酵素化学専攻実験1 Experimental Course of Enzyme Chemistry1 通年 農学研究科 2015/04-2016/03
酵素化学専攻実験 Experimental Course of Enzyme Chemistry 通年 農学研究科 2015/04-2016/03
酵素化学専攻演習1 Enzyme Chemistry Seminar1 通年 農学研究科 2015/04-2016/03
酵素化学専攻演習 Enzyme Chemistry Seminar 通年 農学研究科 2015/04-2016/03
酵素化学特論 Enzyme Chemistry (Advanced Course) 後期集中 農学研究科 2015/04-2016/03
食品分析化学 Analytical Chemistry in Food Science 後期 農学部 2015/04-2016/03
食品生化学I Food Biochemistry I 前期 農学部 2015/04-2016/03
食品生化学II Food Biochemistry II 後期 農学部 2015/04-2016/03
酵素化学 Enzyme Chemistry 後期 農学部 2016/04-2017/03
酵素化学実験及び実験法 Laboratory Course in Enzyme Chemistry 前期 農学部 2016/04-2017/03
酵素化学専攻実験1 Experimental Course of Enzyme Chemistry1 通年 農学研究科 2016/04-2017/03
酵素化学専攻実験2 Experimental Course of Enzyme Chemistry2 通年 農学研究科 2016/04-2017/03
酵素化学専攻実験 Experimental Course of Enzyme Chemistry 通年 農学研究科 2016/04-2017/03
酵素化学専攻演習1 Enzyme Chemistry Seminar1 通年 農学研究科 2016/04-2017/03
酵素化学専攻演習2 Enzyme Chemistry Seminar2 通年 農学研究科 2016/04-2017/03
酵素化学専攻演習 Enzyme Chemistry Seminar 通年 農学研究科 2016/04-2017/03
食品分析化学 Analytical Chemistry in Food Science 後期 農学部 2016/04-2017/03
食品基礎分子生物学II Principles of Molecular Biology in Food Science II 前期 農学部 2016/04-2017/03
食品生物科学概論 Introduction to Food Bioscience 前期 農学部 2016/04-2017/03
基礎生化学II Fundamental Biochemistry II 後期 農学部 2017/04-2018/03
応用酵素化学 Applied Enzyme Chemistry 後期 農学部 2017/04-2018/03
酵素化学 Enzyme Chemistry 後期 農学部 2017/04-2018/03
酵素化学専攻実験1 Experimental Course of Enzyme Chemistry1 通年 農学研究科 2017/04-2018/03
酵素化学専攻実験2 Experimental Course of Enzyme Chemistry2 通年 農学研究科 2017/04-2018/03
酵素化学専攻演習1 Enzyme Chemistry Seminar1 通年 農学研究科 2017/04-2018/03
酵素化学専攻演習2 Enzyme Chemistry Seminar2 通年 農学研究科 2017/04-2018/03
酵素化学特論 Advanced Course of Enzyme Chemistry 前期集中 農学研究科 2017/04-2018/03
食品分析化学 Analytical Chemistry in Food Science 後期 農学部 2017/04-2018/03
食品基礎分子生物学II Principles of Molecular Biology in Food Science II 前期 農学部 2017/04-2018/03
食品生化学実験及び実験法(I) Laboratory Course in Food Biochemistry (I) 前期 農学部 2017/04-2018/03

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School management (title, position)
Title Period
放射線障害予防小委員会 委員 2013/04/01-2015/03/31
学生生活委員会 委員 2017/04/01-2019/03/31
学生生活委員会第一小委員会 委員 2017/04/01-2019/03/31
京都大学奨学金返還免除候補者選考委員会 委員 2017/04/01-2019/03/31
Faculty management (title, position)
Title Period
学生生活委員会 委員 2013/04/01-2015/03/31
制度・組織等検討検討委員会 委員 2015/04/01-2017/03/31
男女共同参画推進委員会 委員 2015/04/01-2017/03/31
食品生物科学科長 2015/04/01-2016/03/31
評価委員会 委員 2015/04/01-2018/03/31
放射線障害防止委員会 委員 2013/04/01-2017/03/31
食品生物科学専攻 専攻長 2016/04/01-2018/03/31
防火・防災委員会 委員 2016/04/01-2018/03/31
研究科教務委員会 委員 2017/04/01-2019/03/31
学部教務委員会 委員 2017/04/01-2019/03/31
人権問題対策委員会 2017/04/01-2019/03/31

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