南 直治郎

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Name(Kanji/Kana/Abecedarium Latinum)
南 直治郎/ミナミ ナオジロウ/Naojiro Minami
Primary department(Org1/Org2/Org3/Job title)
農学研究科/応用生物科学専攻動物遺伝増殖学講座/准教授
Faculty
Org1 Org2 Org3 Job title
農学部
Contact address
Type Address(Japanese) Address(English)
Office 〒606-8502　京都府京都市左京区北白川追分町 Kitashirakawa Oiwake-cho, Kyoto 606-8502, Japan
Affiliated academic organization(s) (Japanese)
Organization name(Japanese) Organization name(English)
日本繁殖生物学会 Society for Reproduction and Development
日本畜産学会 Japanese Society of Animal Science
日本分子生物学会 The Molecular Biology Society of Japan
日本生殖医学会 Japan Society for Reproductive Medicine
日本胚移植研究会 Japan Embryo Transfer Society
Affiliated academic organization(s) (overseas)
Organization name Country
Society for the Study of Reproduction アメリカ
Asian Reproductive Biotechnology Society 日本
Academic degree
Field(Japanese) Field(English) University(Japanese) University(English) Method
農学修士 京都大学
農学博士 京都大学
Graduate school
University(Japanese) University(English) Faculty(Japanese) Faculty(English) Major(Japanese) Major(English) Degree
京都大学 大学院農学研究科修士課程畜産学専攻 修了
京都大学 大学院農学研究科博士後期課程畜産学専攻 修了
University
University(Japanese) University(English) Faculty(Japanese) Faculty(English) Major(s)(Japanese) Major(s)(English) Degree
京都大学 農学部畜産学科（転学部） 卒業
Highschool
Highschool Kana
大分県立宇佐高等学校 おおいたけんりつうさこうとうがっこう
Work Experience
Period Organization(Japanese) Organization(English) Job title(Japanese) Job title(English)
1989/04/01-1991/01/31 1989　日本学術振興会特別研究員 Japan Society for the Promotion of Science 特別研究員 Research Fellow
1991/02/01-2006/04/30 1991　京都大学農学部助手 Kyoto University, Faculty of Agriculture, Instructor 助手 Assistant Professoｒ
2006/05/01- 2006　京都大学農学研究科准教授 Kyoto University, Graduate School of Agriculture, Associate Professor 准教授 Associate Professor
Personal Profile
(Japanese)
　京都大学農学部を卒業後は大学院の修士課程・博士課程に進学し学位を取得しました。その後日本学術振興会特別研究員に採用されましたが、2年の満期を終える直前に農学部の助手として採用されました。修士の学生時代の1984－85年に交換留学生制度を利用し米国ウイスコンシン大学に留学し、Bavister博士の元で、ハムスター初期胚の2-cell Blockの研究に従事し、世界で初めてハムスター初期胚を1細胞期から胚盤胞期胚まで培養することに成功しました。 　助手時代の1994年にも米国カリフォルニア大学ロサンゼルス校に留学する機会を得て、SV40ウイルスの核移行に関する研究に従事しました。 　現在はこれらの経験を生かして、マウス初期胚の発生に関する研究に従事しています。
Research Topics
(Japanese)
ほ乳動物の卵母細胞および初期胚に発現する遺伝子の解析 主にマウス初期胚の発生に関わる遺伝子発現の制御機構をエピジェネティクスの観点から研究しています。
(English)
Studies on gene expression in mammalian oocytes and preimplantation embryos
Overview of your research
(Japanese)
　ほ乳動物初期胚の発生は受精によってスタートするが、受精前では卵母細胞、精子とも遺伝子発現が停止した状態である。受精を経て初期胚は自ら遺伝子発現を開始するが、これは胚性ゲノムの活性化と呼ばれこの活性化が起こるまでは、卵母細胞内に蓄えられた転写産物やタンパク質によって発生が制御されている。これらの遺伝子の中には卵母細胞特異的に発現を開始するが、受精後の初期発生で機能する遺伝子がいくつか同定されており、母性効果遺伝子と呼ばれている。これらの遺伝子を含め、卵母細胞および初期胚で発現する遺伝子が初期発生をどのように制御しているか、また胚性ゲノムの活性化やそれ以降に発生を制御する遺伝子がどのように発現制御させているかをエピジェネティクスの観点から研究している。
(English)
Development in mammals starts with fertilization. Before fertilization gene expression is cmpletly silenced in both oocytes and spermatozoa. Several hours after fiertilization gene expression starts in zygotic genome in the mouse and the first gene expression after fertilization is called zygotic gene expression (ZGA). Until that moment development is controlled by the factors accumulated in oocytes. Several oocyte-specific genes are identified to function only after fertilization and these genes are called maternal effect genes.My research projects are to investigate how do the genes expressed oocyte-specifically or the genes expressed after fertilization function to controll preimplantation development and to clarify how does the gene expression at ZGA or after fertilization controlled epigenetically.
Fields of research (key words)
Key words(Japanese) Key words(English)
生殖生物学
遺伝子発現
卵母細胞
初期胚
胚性ゲノムの活性化
Fields of research (kaken code)
Kaken code
Applied animal science
Published Papers > Published Papers
(文系研究者の一般的な論文集への寄稿や単行本の分担執筆などは「著書等」を参照してください)
Authors Title Bibliography Date Peer-Review Language
Ohta Y, Nagao Y, Minami N, Tsukamoto S, Kito S. Deficiencies in extrusion of the second polar body due to high calcium concentrations during in vitro fertilization in inbred C3H/He mice. Zygote 24: 603-616. doi:10.1017/S096719941500060X. 2015/09 Refereed English
Suzuki S, Nozawa Y, Tsukamoto S, Kaneko T, Manabe I, Imai S, Minami N. CHD1 acts via the Hmgpi pathway to regulate mouse early embryogenesis. Development, 2015 Jul 1; 142: 2375-2384; posted ahead of print June 19, 2015, doi:10.1242/dev.120493. 2015/07 Refereed English
Suzuki S, Nozawa Y, Tsukamoto S, Kaneko T, Imai S, Minami N. Histone methyltransferase Smyd3 regulates early embryonic lineage commitment in the mouse. Reproduction, 2015 Jul 1; 150(1): 21-30. doi: 10.1530/REP-15-0019. 2015/07 Refereed English
S. Goel; M. Fujihara; K. Tsuchiya; Y. Takagi; N. Minami; M. Yamada; H. Imai Multipotential ability of primitive germ cells from neonatal pig testis cultured in vitro Reproduction, Fertility and Development,21,5,696-708 2009 Refereed English
塚本智史・今市寿史・木村康二・今井　裕・南　直治郎 ウシ胚盤胞期胚のcDNAライブラリー構築法 日本胚移植学雑誌 = Japanese journal of embryo transfer,28,3,118-123 2006/09/22 Refereed Japanese
岩田 尚孝・赤松 真一・南 直治郎 斉一な牛群を用いて作製した体外受精・体外発育卵と体内受精・体内発育卵に由来する産子体重の比較 日本繁殖生物学会誌,44/,33-38 1998 Refereed Japanese
南 直治郎 ホ乳動物初期胚の発生におよぼす卵管の影響 受精着床学会誌,11/,26-27 1994 Refereed Japanese
南 直治郎 耐凍剤および凍結融解法が-196℃に保存された家兎桑実胚の生存性に及ぼす影響 家畜繁殖学雑誌,30,1,46-49 1984/03 Refereed Japanese
Kawaguchi T, Tsukiyama T, Kimura K, Matsuyama S, Minami N, Yamada M, Imai H. Generation of Naive Bovine Induced Pluripotent Stem Cells Using piggyBac Transposition of Doxycycline-inducible Transcription Factors PLoS ONE,10,8,e0135403-naojiro 2015/08 Refereed English
S.-M. Kim; M. Fujihara; M. Sahare; N. Minami; M. Yamada; H. Imai Effects of extracellular matrices and lectin Dolichos biflorus agglutinin on cell adhesion and self-renewal of bovine gonocytes cultured in vitro Reproduction, Fertility and Development,26,2,268-281 2014 Refereed English
S. Tsukamoto; T. Hara; A. Yamamoto; S. Kito; N. Minami; T. Kubota; K. Sato; T. Kokubo Fluorescence-based visualization of autophagic activity predicts mouse embryo viability Scientific Reports,4 2014 Refereed English
S. Suzuki; Y. Nozawa; S. Tsukamoto; T. Kaneko; H. Imai; N. Minami ING3 Is Essential for Asymmetric Cell Division during Mouse Oocyte Maturation PLoS ONE,8,9,e74749 2013/09/16 Refereed English
M. Ishida; E. Okazaki; S. Tsukamoto; K. Kimura; A. Aizawa; S. Kito; H. Imai; N. Minami The Promoter of the Oocyte-Specific Gene, Oog1, Functions in Both Male and Female Meiotic Germ Cells in Transgenic Mice PLoS ONE,8,7,e68686 2013/07/22 Refereed English
Kim SM, Fujihara M, Sahare M, Minami N, Yamada M, Imai H Effects of extracellular matrices and the lectin DBA on cell adhesion and self-renewal of bovine gonocytes cultured Reprod Fert Dev, doi: 10.1071/RD12214 (in press) 2013 Refereed English
TSUKAMOTO Satoshi;HARA Taichi;YAMAMOTO Atsushi;YAMAMOTO Atsushi;OHTA Yuki;WADA Ayako;ISHIDA Yuka;KITO Seiji;NISHIKAWA Tetsu;MINAMI Naojiro;SATO Ken;KOKUBO Toshiaki Functional Analysis of Lysosomes During Mouse Preimplantation Embryo Development J Reprod Dev,59,1,33-39 (J-STAGE)-39 2013 Refereed English
T. Tsukiyama; R. Asano; T. Kawaguchi; N. Kim; M. Yamada; N. Minami; Y. Ohinata; H. Imai Simple and efficient method for generation of induced pluripotent stem cells using piggyBac transposition of doxycycline-inducible factors and an EOS reporter system Genes to Cells,16,7,815-825 2011/07 Refereed English
M. Fujihara; S.-M. Kim; N. Minami; M. Yamada; H. Imai Characterization and in vitro culture of male germ cells from developing bovine testis Journal of Reproduction and Development,57,3,355-364 2011/06 Refereed English
K. Miyamoto; T. Tsukiyama; Y. Yang; N. Li; N. Minami; M. Yamada; H. Imai Cell-free extracts from mammalian oocytes partially induce nuclear reprogramming in somatic cells Biology of Reproduction,80,5,935-943 2009/05 Refereed English
K. Miyamoto; T. Yamashita; T. Tsukiyama; N. Kitamura; N. Minami; M. Yamada; H. Imai Reversible membrane permeabilization of mammalian cells treated with digitonin and its use for inducing nuclear reprogramming by Xenopus egg extracts Cloning and Stem Cells,10,4,535-542 2008/12 Refereed English
S. Goel; M. Fujihara; N. Minami; M. Yamada; H. Imai Expression of NANOG, but not POU5F1, points to the stem cell potential of primitive germ cells in neonatal pig testis Reproduction,135,6,785-795 2008/06 Refereed English
M. Fujihara; S. Goel; N. Minami; M. Yamada; H. Imai Cryopreservation in liquid nitrogen of gonocytes from neonatal porcine testes stored at 4°C Reproductive Medicine and Biology,7,4,153-160 2008 Refereed English
T. Watanabe; H. Imai; N. Minami Identification and expression analysis of small RNAs during development Methods in Molecular Biology,442,173-185 2008 Refereed English
K. Miyamoto; T. Furusawa; M. Ohnuki; S. Goel; T. Tokunaga; N. Minami; M. Yamada; K. Ohsumi; H. Imai Reprogramming events of mammalian somatic cells induced by Xenopus laevis egg extracts Molecular Reproduction and Development,74,10,1268-1277 2007/10 Refereed English
N. Minami; T. Suzuki; S. Tsukamoto Zygotic gene activation and maternal factors in mammals Journal of Reproduction and Development,53,4,707-715 2007/08 Refereed English
S. Goel; M. Sugimoto; N. Minami; M. Yamada; S. Kume; H. Imai Identification, isolation, and in vitro culture of porcine gonocytes Biology of Reproduction,77,1,127-137 2007/07 Refereed English
T. Suzuki; N. Minami; T. Kono; H. Imai Comparison of the RNA polymerase I-, II- And III-dependent transcript levels between nuclear transfer and in vitro fertilized embryos at the blastocyst stage Journal of Reproduction and Development,53,3,663-671 2007/06 Refereed English
K. Miyamoto; Y. Hoshino; N. Minami; M. Yamada; H. Imai Effects of synchronization of donor cell cycle on embryonic development and DNA synthesis in porcine nuclear transfer embryos Journal of Reproduction and Development,53,2,237-246 2007/04 Refereed English
T. Watanabe; Y. Totoki; H. Sasaki; N. Minami; H. Imai Analysis of Small RNA Profiles During Development Methods in Enzymology,427,155-169 2007 Refereed English
M. Ji; N. Minami; M. Yamada; H. Imai Effect of protopanaxatriol saponin on spermatogenic stem cell survival in busulfan-treated male mice Reproductive Medicine and Biology,6,2,99-108 2007 Refereed English
T. Watanabe; A. Takeda; T. Tsukiyama; K. Mise; T. Okuno; H. Sasaki; N. Minami; H. Imai Identification and characterization of two novel classes of small RNAs in the mouse germline: Retrotransposon-derived siRNAs in oocytes and germline small RNAs in testes Genes and Development,20,13,1732-1743 2006/07/01 Refereed English
S. Tsukamoto; R. Ihara; A. Aizawa; S. Kishida; A. Kikuchi; H. Imai; N. Minami Oog1, an oocyte-specific protein, interacts with Ras and Ras-signaling proteins during early embryogenesis Biochemical and Biophysical Research Communications,343,4,1105-1112 2006/05/19 Refereed English
T. Suzuki; N. Minami; T. Kono; H. Imai Zygotically activated genes are suppressed in mouse nuclear transferred embryos Cloning and Stem Cells,8,4,295-304 2006 Refereed English
N. Minami; S. Tsukamoto Role of oocyte-specific genes in the development of mammalian embryos Reproductive Medicine and Biology,5,3,175-182 2006 Refereed English
T. Watanabe; A. Takeda; K. Mise; T. Okuno; T. Suzuki; N. Minami; H. Imai Stage-specific expression of microRNAs during Xenopus development FEBS Letters,579,2,318-324 2005/01/17 Refereed English
Y. Hoshino; M. Uchida; Y. Shimatsu; M. Miyake; Y. Nagao; N. Minami; M. Yamada; H. Imai Developmental competence of somatic cell nuclear transfer embryos reconstructed from oocytes matured in vitro with follicle shells in miniature pig Cloning and Stem Cells,7,1,17-26 2005 Refereed English
A. Ohashi; H. Imai; N. Minami Cyclin A2 Is Phosphorylated During the G2/M Transition in Mouse Two-Cell Embryos Molecular Reproduction and Development,66,4,343-348 2003/12 Refereed English
N. Minami; A. Aizawa; R. Ihara; M. Miyamoto; A. Ohashi; H. Imai Oogenesin Is a Novel Mouse Protein Expressed in Oocytes and Early Cleavage-Stage Embryos Biology of Reproduction,69,5,1736-1742 2003/11 Refereed English
D. Kigami; N. Minami; H. Takayama; H. Imai MuERV-L is one of the earliest transcribed genes in mouse one-cell embryos Biology of Reproduction,68,2,651-654 2003/02 Refereed English
H. Iwata; K. Kimura; S. Hashimoto; M. Ohta; K. Tominaga; N. Minami Role of G6PD activity on sex ratio and developmental competence of bovine bnbryos under oxidative stress Journal of Reproduction and Development,48,5,447-453 2002/10 Refereed English
S. Kurosaka; Y. Nagao; N. Minami; M. Yamada; H. Imai Dependence of DNA synthesis and in vitro development of bovine nuclear transfer embryos on the stage of the cell cycle of donor cells and recipient cytoplasts Biology of Reproduction,67,2,643-647 2002/08 Refereed English
S. Hashimoto; N. Minami; R. Takakura; H. Imai Bovine immature oocytes acquire developmental competence during meiotic arrest in vitro Biology of Reproduction,66,6,1696-1701 2002/06 Refereed English
A. Ohashi; N. Minami; H. Imai Nuclear accumulation of cyclin B1 in mouse two-cell embryos is controlled by the activation of Cdc2 Biology of Reproduction,65,4,1195-1200 2001/10 Refereed English
N. Minami; K. Sasaki; A. Aizawa; M. Miyamoto; H. Imai Analysis of gene expression in mouse 2-cell embryos using fluorescein differential display: Comparison of culture environments Biology of Reproduction,64,1,30-35 2001/01 Refereed English
S. Hashimoto; N. Minami; R. Takakura; M. Yamada; H. Imai; N. Kashima Low oxygen tension during in vitro maturation is beneficial for supporting the subsequent development of bovine cumulus-Oocyte complexes Molecular Reproduction and Development,57,4,353-360 2000/12 Refereed English
S. Hashimoto; N. Minami; M. Yamada; H. Imai Excessive concentration of glucose during in vitro maturation impairs the developmental competence of bovine oocytes after in vitro fertilization: Relevance to intracellular reactive oxygen species and glutathione contents Molecular Reproduction and Development,56,4,520-526 2000/08 Refereed English
S. Hashimoto; R. Takakura; M. Yoshinari; N. Minami; M. Yamada; H. Imai; N. Kashima Relationship between the Responsiveness to Multiple-ovulation Treatment and the Number of Bovine Oocytes Collected by Transvaginal Follicle Aspiration Journal of Veterinary Medical Science,62,6,647-650 2000/06 Refereed English
Iwata H, Minami N, Imai H. Postnatal weights of calves derived from IVM/IVF embryos developed under various oxygen concentrations. Reprod. Fert. Dev.,/12, 391-396 2000 Refereed English
S. Hashimototo; R. Takakura; N. Minami; M. Yamada Ultrasound-guided follicle aspiration: Effect of the frequency of a linear transvaginal probe on the collection of bovine oocytes Theriogenology,52,1,131-138 1999/07/01 Refereed English
S. Hashimoto; R. Takakura; M. Kishi; T. Sudo; N. Minami; M. Yamada Ultrasound-guided follicle aspiration: The collection of bovine cumulus- oocyte complexes from ovaries of slaughtered or live cows Theriogenology,51,4,757-765 1999/03 Refereed English
H. Iwata; S. Akamatsu; N. Minami; M. Yamada Allopurinol, an inhibitor of xanthine oxidase, improves the development of IVM/IVF bovine embryos (>4 cell) in vitro under certain culture conditions Theriogenology,51,3,613-622 1999/02 Refereed English
H. Iwata; S. Akamatsu; N. Minami; M. Yamada Effects of antioxidants on the development of bovine IVM/IVF embryos in various concentrations of glucose Theriogenology,50,3,365-375 1998/08 Refereed English
S. Hashimoto; K. Saeki; Y. Nagao; N. Minami; M. Yamada; K. Utsumi Effects of cumulus cell density during in vitro maturation on the developmental competence of bovine oocytes Theriogenology,49,8,1451-1463 1998/06 Refereed English
S. Hashimoto; K. Saeki; Y. Nagao; N. Minami; M. Yamada; K. Utsumi Erratum: Effects of cumulus cell density during in vitro maturation on the developmental competence of bovine oocytes (An International Journal of Animal Reproduction (1998) 49 (1451-1463)) Theriogenology,50,2,334-335 1998 Refereed English
A. Nishikimi; M. Yamada; N. Minami; K. Utsumi Evaluation of acrosomal status of bovine spermatozoa using concanavalin a lectin Theriogenology,48,6,1007-1016 1997/10/15 Refereed English
K.S. Kim; N. Minami; M. Yamada; K. Utsumi Follicular cells affect the fertilizability and developmental competency of bovine oocytes in vitro Reproduction, Fertility and Development,9,8,763-766 1997 Refereed English
N. Minami Early embryonic development under oviductal influence in vitro Animal Reproduction Science,42,1月4日,361-369 1996/04 Refereed English
N. Ishii; N. Minami; E.Y. Chen; A.L. Medina; M.M. Chico; H. Kasamatsu Analysis of a nuclear localization signal of simian virus 40 major capsid protein Vp1 Journal of Virology,70,2,1317-1322 1996/02 Refereed English
Hosoi Y, Minami N and Iritani A Endocrine Regulation of Early Embryonic Development and Implantation Hormone Research,44, 9-14 1995 Refereed English
N. Minami; H. Kato; Y. Inoue; M. Yamada; K. Utsumi; A. Iritani Nonspecies-specific effects of mouse oviducts on the development of bovine IVM/IVF embryos by a serum free co-culture Theriogenology,41,7,1435-1445 1994/05 Refereed English
N. Minami; A. Iritani Role of the oviduct in the development of the mouse embryo Molecular Reproduction and Development,36,2,279-281 1993/10 Refereed English
N. Minami; K. Utsumi; A. Iritani Effects of low molecular weight oviductal factors on the development of mouse one-cell embryos in vitro Journal of Reproduction and Fertility,96,2,735-745 1992/11 Refereed English
N. Minami; B.D. Bavister; A. Iritani Development of hamster two-cell embryos in the isolated mouse oviduct in organ culture system Gamete Research,19,3,235-240 1988/03 Refereed English

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Conference Presentation > Conference Presentation
(文系研究者の一般的な学会報告の業績は「講演等」を参照してください)
Authors Title Bibliography Date Peer-Review Language
Tsukamoto S, Hara T, Sato K, Minami N, Kiro S, Tsukamoto S. In vivo analysis of processing bodies using transgenic mice expressing GFP-Dcp1a 15th International Congress of Radiation Research 2015/05 English
Miyamoto Y, Kawahara Y, Suzuki S, Ishida M, Tsukamoto S, Imai H, Minami N. The role of Oog1 in female meiotic germ cells: Activation of oocyte-specific genes and suppression of testis-associated genes. The 10th Annual Conference of the Asian Reproductive Biotechnology Society 2014/11 English
Suzuki, S, Nozawa Y, Tsukamoto S, Kaneko T, Imai H, Minami N. CHD1 is the zygotic determinant of Oct4 and Cdx2 expression via the Hmgpi pathway during preimplantation development. 2014 World Congress of Reproductive Biology 2014/09 English
Miyamoto Y, Kawahara Y, Suzuki S, Ishida M, Tsukamoto S, Imai H, Minami N. Oog1 functions during meiosis of female germ cells through suppression of testis-associated genes and activation of oocyte-specific genes. The 47th Annual Meeting of the Society for the Study of Reproduction 2014/08 English
Tsukamoto S, Hara T, Yamamoto A, Kito S, Minami N,Sato K, Kokubo T Monitoring of autophagic activity in the developing mouse preimplantation embryo. The 5th Europian Molecular Biology Organaization Meeting 2013 Refereed English
Suzuki S, Nozawa Y, Tsukamoto S, Kaneko T, Imai H, Minami N Ing3 has an important role during oocyte maturation in the mouse The 46th Annual Meeting of the Society for the Study of Reproduction 2013 Refereed English
Luong PQ, Matsumoto M, Suzuki S, Kitamura N, Miyamoto K, Minami N, Yamada M and Imai H The roles of Hsp90 in reprogramming process in mouse preimplantation embryonic development CiRA International Symposium 2013 Refereed English
Kim SM, Fujihara M, Goel S, Suzuki S, Minami N, Yamada M and Imai H Glycan epitopes involve in germ cell differentiation during early development of neonatal mice testis 4th Congress of the Asia Pacific Initiaive on Reproduction 2012 Refereed English
Ishida M, Okazaki E, Tsukamoto S, Kimura K, Ohota Y, Kito S, Imai H and Minami N Oog1 promoter controls the meiosis-specific gene expression in germ cells The 45th Annual Meeting of the Society for the Study of Reproduction 2012 Refereed English
Kim SM, Fujihara M, Goel S, Suzuki S, Minami N, Yamada M and Imai H Determination of distinct germ cell commitment by the specific affinity of the lectin dba during early development of gonocytes in neonatal mice testis International Congress of Animal Reproduction 2012 Refereed English
Suzuki S, Nozawa Y, Tsukamoto S, Kaneko T, Imai H, Minami N Effects of chromatin remodeling factor Chd1 on in vitro differentiation of mouse preimplantation embryos 10th Annual Meetinf of International Socirety for Stem Cell Reseach 2012 Refereed English
Suzuki S, Tsukamoto S, Ohota Y, Imai Hiroshi, Minami N The functional analysis of Chd1 in mouse oocyte maturation using lipid-siRNA complexes Joint Meeting of the Brithish Society for Cell Biology, Developmental Biology and the Japanese Society for Developmental Biologists 2012 Refereed English
K. Miyamoto; K. Nagai; N. Kitamura; T. Nishikawa; H. Ikegami; N.T. Binh; S. Tsukamoto; M. Matsumoto; T. Tsukiyama; N. Minami; M. Yamada; H. Ariga; M. Miyake; T. Kawarasaki; K. Matsumoto; H. Imai Identification and characterization of an oocyte factor required for development of porcine nuclear transfer embryos Proceedings of the National Academy of Sciences of the United States of America,108,17,7040-7045 2011/04/26 Refereed English
Miya Ishida, Eriko Okazaki, Satoshi Tsukamoto, Koji Kimura, Yuki Ohta, Seiji Kito, Hiroshi Imai and Naojiro Minami Identification of Oog1 promoter regions in mouse growing oocytes 44th Society for the Study of Reproduction 2011 Refereed English
Okazaki, Eriko; Tsukamoto, Satoshi; Kimura, Koji; Ohta, Yuki; Kito, Seiji; Imai, Hiroshi; Minami, Naojiro Identification of Oog1 Promoter Regions which Function During the First Meiosis in Oocytes. BIOLOGY OF REPRODUCTION,170-170 2010 Refereed English
Imaichi H, Okazaki E, Tsukamoto S, Ohota Y, Kito S, Kimura K, Imai H, Minami N ZP3 promoter is not enough to analyze the early expressing genes during oogenesis, such as Oog1 Analysis of Oog1, an oocyte-specific gene, using transgenic RNAi approach. 42nd Society for the Study of Reproduction 2009 Refereed English
Tsukiyama, Tomoyuki; Miyamoto, Kei; Minami, Naojiro; Yamada, Masayasu; Imai, Hiroshi Reprogramming of mouse embryonic fibroblasts by cell-free extracts from nanog-overexpressing embryonic stem cells BIOLOGY OF REPRODUCTION,85-85 2008 Refereed English
Minami N, Hisashi Imaich, Tsukamoto S, Ohota Y, Kito S, Kimura K and Imai H Oog1 is a maternal effect gene required for the development of mouse preimplantation embryo. 16th International Congress on Animal Reproduction 2008 Refereed English
Miyamoto, K., Tsukiyama, T., Yang, Y., Li, N., Minami N., Yamada, M., Imai, H. DNA demethylation associated with nuclear reprogramming of the somatic cell genome in cell-free extracts from mammalian oocytes. 41st Society for the Study of Reproduction 2008 Refereed English
Tsukamoto S, Imaichi H, Kito S, Ohota Y, Aizawa A, Imai H and Minami N Investigation of Oog1, an oocyte-specific gene, using RNAi approach 40th Society for the Study of Reproduction 2007 Refereed English
Miyamoto K, Ohnuki M, Minami N, Yamada M and Imai H Nuclear reprogramming of porcine cells and their use as donor cell for nuclear transfer after treatment in Xenopus egg extracts 33rd International Embryo Transfer Society 2007 Refereed English
Goel S, Sugimoto M, Kume S, Yamada M, Minami N and Imai H Lectin Dolichos Biflourus Agglutinin (DBA) is a specific marker for primitive germ cells in neonatal porcine testis 33rd International Embryo Transfer Society 2007 Refereed English
Kato S, Mukojima K, Otani T, Sakai K, Tamura K, Miyazaki K, Minami N and Imai H Management system for frozen bull semen using a radio frequency identification tag. 33rd International Embryo Transfer Society 2007 Refereed English
Miyamoto K, Tsukiyama T, Minami N, Yamada M, Imai H. Differential nuclear reprogramming of somatic cells by porcine germinal vesicle and metaphase II oocyte extracts. The 4th Asian Reproductive Biotechnology Conference 2007 Refereed English
Goel S, Fujihara M, Minami N, Yamada M, Imai H NANOG is expressed by progenitor-type stem cells and spermatocytes in porcine testis. The 4th Asian Reproductive Biotechnology Conference 2007 Refereed English
Fujihara M, Goel S, Kimura Y, Minami N, Yamada M, Imai H. Preservation of gonocytes in pigs. The 4th Asian Reproductive Biotechnology Conference 2007 Refereed English
Suzuki T, Minami N, Imai H Zygotically activated genes are suppressed in mouse nuclear transferred embryos 33rd International Embryo Transfer Society 2007 Refereed English
SUZUKI Toru;MINAMI Naojiro;IMAI Hiroshi The quantitative aberration of gene epression in the mouse NT embryos at the 2-cell stage 生化学,A10512(5P-A-337) 2006 Refereed English
Watanabe T, Takeda A, Tsukiyama T, Sasaki H, Minami N and Imai H Two novel classes of small RNAs from mouse germline International Genomic Imprinting Workshop 2006 Refereed English
Watanabe T, Tsukiyama T, Sasaki H, Minami N and Imai H Identification and characterization of two novel classes of small RNAs in the mouse germ line Mouse Molecular Genetics Cold Spring Harbor Laboratory 2006 Refereed English
Tsukamoto S, Imaichi H, Kito S, Ohota Y, Aizawa A, Imai H and Minami N Functional analysis of Oog1, an oocyte-specific gene, in mouse embryo The 3rd Asian Reproductive Biotechnology Conference 2006 Refereed English
Miyamoto K, Ohnuki M, Minami N, Yamada M and Imai H Reprogramming of intact and permeabilized mammalian somatic cells by Xenopus egg extract The 3rd Asian Reproductive Biotechnology Conference 2006 Refereed English
Tsukamoto S, Ihara R, Aizawa A, Nakanishi A, Imai H and Minami N Oog1, an oocyte-specific protein, interact with Ran during the first mitosis in mouse embryo. 39th Society for the Study of Reproduction 2006 Refereed English
Suzuki T, Minami N and Imai H Suppressive expression of zygotically activated genes in mouse cloned embryos. 39th Society for the Study of Reproduction 2006 Refereed English
Tsukamoto S, Aizawa A, Nakanishi A, Kishida S, Kikuchi A, Imai H and Minami N Functional analysis of Oog1, an oocyte-specific gene, in mouse embryo. 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress 2006 Refereed English
Park J-S, Minami N and Imai H Comprehensive identification of relative genes causing abnormal development of bovine embryos produced by somatic cell nuclear transfer. 32nd International Embryo Transfer Society 2006 Refereed English
Miyamoto K, Nagao Y, Minami N, Yamada M, Ohsumi K and Imai H Nuclear reprogramming of porcine fibroblast cells by Xenopus egg extracts. 32nd International Embryo Transfer Society 2006 Refereed English
Tsukamoto S, Ihara R, Aizawa A, Imai H and Minami N Involvement of an oocyte-specific novel gene Oog1 in zygotic genome activation. 38th Society for the Study of Reproduction 2005 Refereed English
Suzuki, T; Minami, N; Imai, H Single embryo analysis of the gene expression in the mouse 2-cell stage embryos reconstructed by somatic nuclear transfer. BIOLOGY OF REPRODUCTION,240-241 2004 Refereed English
Park J-S, Minami N and Imai H Analysis of gene expression of bovine embryos produced by somatic cell nuclear transfer. 15th International Congress of Animal Reproduction 2004 Refereed English

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Lectures > Invited Lectures
(文系研究者の一般的な学会報告はこの項に収録されております)
Title Conference name Organization name Date Language
CHD1 is a zygotic determinant for cell lineage specification via the HMGPI pathway in mouse preimplantation embryo. [Invited] 49th Annual Meeting of the Society for the Study of Reproduction 2016/07/18 English
初期胚の発生と遺伝子 第27回受精着床学会 受精着床学会 2010 Japanese
卵子に発現する遺伝子Oog1 第2回学術シンポジウム「動物生命工学の新潮流」－クローン・生殖・再生への挑戦－ 近畿大学 2008 Japanese
Regulation of meiosis-specific gene expression by the promoter of an oocyte-specific gene, Oog1 The 9th Annual Conference of the Asian Reproductive Biotechnology Society Asian Reproductive Biotechnology Society 2012 English
Meiosis-specific gene expression by the promoter of an oocyte-specific gene, Oog1 [Invited] 2012 CABX Workshop and International Symposium The Center for the Animal Bio-Reactor and Xenotransplantation 2012 English
Role of Oog1, a novel maternal effect gene in the development of mouse embryo The 4th Asian Reproductive Biotechnology Conference Asian Reproductive Biotechnology Society 2007 English
A novel maternal effect gene, Oog1: Involvement in zygotic gene activation and early embryonic development. [Invited] Workshop on “Mammalian Oogenesis and Epigenetic Modification” 2005 English
A novel maternal effect gene, Oogenesin: Involvement in zygotic gene activation and early embryonic development in the mouse The 5th Royan International Research Congress 2004 English
Publications > Books
Authors Title Publisher Date Language
南　直治郎 卵子学 京都大学学術出版会 2011 Japanese
Watanabe, T., Imai, H. and Minami N. Identification and Expression Analysis of Small RNAs During Development Methods Mol Bio. 2008; 442: 173-185. 2008 English
A. Ohashi and N. Minami Nuclear accumulation of cyclin B1 in mouse 2-cell embryos: Involvement of the activation of Cdc2. In "Reproductive Biotechnology and Relating Physiolog Hokuto Shobo,297-301 2001 English
S. Hashimoto and N. Minami In vitro maturation of bovine oocytes under low oxygen tension. In "Reproductive Biotechnology and Relating Physiology" Hokuto Shobo,229-233 2001 English
S. Kurosaka, N. Minami and H. Imai Cytoplasmic environment and cell cycle co-ordination of recipient and donor cells in nuclear transfer embryos. In "Reproductive Biotechnology and Relating Physiolog Hokkuto Shobo,267-271 2001 English
N. Minami Gene expression and developmental competence of mouse 2-cell embryos cultured in vitro. In "Reproductive Biotechnology and Relating Physiology", (Miyamoto, H. and Manabe, N. eds.) Hokuto Shobo,155-160 2001 English
M. Yamada, A. Nishikimi, N. Uekawa, K. Hoshino and N. Minami Extraembryonic environment and development of preimplantation rat embryos. In "Reproductive Biology Update-Novel Tools for Assessment of Environmental Toxicity-", (Miyamoto, H. and Manabe, N id. ). Shoukadoh Booksellers Company,/, 355-364 1998 English
A. Nishikimi, J. Mukai, N. Minami and M. Yamada Expression and activation of nuclear factor kappa B during mouse preimplantation development. In "Reproductive Biology Update-Novel Tools for Assessment of Environmental Toxicity-", (Miyamoto, H. and Manabe, N ed. ). Shoukadoh Booksellers Company,/, 183-187 1998 English
R. Parnpai, N. Minami and M. Yamada Current status of research for the establishment of bovine embryonic stem (ES) cells. In "Reproductive Biology Update-Novel Tools for Assessment of Environmental Toxicity-", (Miyamoto, H. and Manabe, N ed. ). Shoukadoh Booksellers Company,/, 383-388 1998 English
N. Minami, A. Ohashi, K. Sasaki, T. Wada and M. Yamada Second cell cycle and cell adhesion of mouse embryos developed in an oviductal environment in vitro. In "Reproductive Biology Update-Novel Tools for Assessment of Environmental Toxicity-", (Miyamoto, H. and Manabe, N ed. ). Shoukadoh Booksellers Company,/, 345-353 1998 English
Patents
Right holders Title Stage Patent number Date
無線ICタグ封入ストロー 特許出願 特願特許出願2006-264064
Awards
Title(Japanese) Title(English) Organization name(Japanese) Organization name(English) Date
The 5th Royan International Research Award Novel Maternal Effect Gene, Oogenesin: Involvement in Zygotic Gene Activation and Early Embryonic Development in The Mouse Royan Institute 2004
Newspaper articles
Title Media name Column name Date
受精卵の発生左右する遺伝子の働き発見 京都新聞 教育欄 2015/07/25
体外受精の受胎確率判別 日経新聞 社会面 2015/06/30
External funds, competitive funds and Grants-in-Aid for Scientific Research(kaken)
Type Position Title(Japanese) Title(English) Period
基盤研究B Representative 胚性ゲノムの活性化から着床までのエピジェネティクスと胚の全能性 2016/04/01-2019/03/31
挑戦的萌芽研究 Representative CRISPR/Cas9システムを利用したほ乳動物の産み分けに関する研究 （平成27年度分） 2014/04/01-2016/03/31
挑戦的萌芽研究 Representative 受精阻害遺伝子を利用したほ乳動物の産み分けに関する研究 2012-2013
基盤研究（B） Representative 母性因子による胚性ゲノム活性化のエピジェネティクス解析 2011-2013
挑戦的萌芽研究 Representative 精子形成期の半数体特異的遺伝子発現を利用した単性動物の作出 2009-2010
基盤研究（B） Representative 卵子特異的遺伝子Oog1の機能解析と胚性ゲノム活性化の分子基盤 Functional analysis of oocyte-specific gene, oog1 and molecular basis of zygotic gene activation 2007-2009
萌芽研究 Representative ほ乳類精子形成期の半数体特異的発現遺伝子を利用した単性動物の創造 2007-2008
基盤研究（B） Representative 胚性ゲノムの活性化時期に核移行する新規遺伝子Oogenesinの機能解析 2004-2006
基盤研究(C) Representative 減数分裂期に卵母細胞特異的に発現を開始する新規遺伝子c-1の機能解析 2002-2003
基盤研究(C) Assignment ミトコンドリアDNAを置換した胚性幹細胞株のキメラ形成能に関する研究 2000-2001
基盤研究(C) Representative 哺乳動物初期胚の細胞周期と遺伝子発現を調節する卵管環境に関する研究 1998-1999
科学研究費基盤研究(A) Assignment 超音波画像誘導による生体回収ウシ卵子の発育能力と遺伝子発現に関する研究 1996-1998
科学研究費基盤研究(A) Assignment 哺乳動物初期胚の遺伝子発現と発生に関する分子機構 1996-1998
科学研究費萌芽的研究 Assignment 精子ベクター法の開発-核移行およびDNA結合シグナルペプチドの応用- 1996-1997
科学研究費一般研究C Representative 哺乳動物初期胚の発生と分化に関与する細胞接着および細胞間情報伝達機構に関する研究 1995-1996
科学研究費一般研究C Assignment 哺乳動物着床前期胚の分割における胚細胞表面糖鎖とその修飾物質の役割 1995-1996
科学研究費奨励研究(A) Representative 哺乳動物初期胚の発生に関与する卵管因子と細胞接着に関する研究 1994-1995
科学研究費試験研究(B) Assignment 遺伝子増幅法による性分別ウシ胚の移植システムの開発 1993-1995
科学研究費一般研究C Assignment 哺乳動物着床前胚の分割における細胞表面糖鎖とその修飾物質の役割 1993-1995
科学研究費奨励研究(A) Representative 卵管由来因子とマウス初期胚の発生過程に形成されるGap Junctionについて 1993-1994
科学研究費一般研究(B) Assignment 哺乳動物における性分化遺伝子による雄性分化機構の解析に関する研究 1992-1994
科学研究費奨励研究(A) Representative マウス初期胚の発生に関与する卵管由来因子に関する研究 1992-1993
日本学術振興会奨励研究 Representative 哺乳動物初期胚の体外培養に関する研究 1989-1991

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Teaching subject(s)
Name(Japanese) Name(English) Term Department Period
細胞生物学II -
昆虫・魚・哺乳動物の生理学 -
生殖生物学入門　–ほ乳類の受精と初期発生- -
動物生理学 前期 農学部 2011/04-2012/03
細胞生物学II 前期 農学部 2011/04-2012/03
分子発生学特論 農学研究科 2011/04-2012/03
生殖生物学専攻実験 農学研究科 2011/04-2012/03
生殖生物学演習 農学研究科 2011/04-2012/03
動物生理学 Physiology 前期 農学部 2012/04-2013/03
昆虫・魚・哺乳動物の生理学 Comparative Physiology 前期 全学共通科目 2012/04-2013/03
細胞生物学Ⅱ Cell Biology II 前期 農学部 2012/04-2013/03
細胞生物学Ⅱ Cell Biology II 前期 農学部 2013/04-2014/03
分子発生学特論 Molecular Embryology in Mammals 前期 農学研究科 2013/04-2014/03
生殖生物学演習 Seminar for Reproductive Biology 通年 農学研究科 2013/04-2014/03
生殖生物学専攻実験 Laboratory Course for Reproductive Biology 通年 農学研究科 2013/04-2014/03
昆虫・魚・哺乳動物の生理学 Comparative Physiology 前期 全学共通科目 2013/04-2014/03
細胞生物学Ⅱ Cell Biology II 前期 農学部 2014/04-2015/03
生殖生物学演習 Seminar for Reproductive Biology 通年 農学研究科 2014/04-2015/03
生殖生物学専攻実験 Laboratory Course for Reproductive Biology 通年 農学研究科 2014/04-2015/03
昆虫・魚・哺乳動物の生理学 Comparative Physiology 前期 全学共通科目 2014/04-2015/03
分子発生学特論 Molecular Embryology in Mammals 前期 農学研究科 2015/04-2016/03
動物生理学 Physiology 前期 農学部 2015/04-2016/03
生殖生物学専攻実験１ Laboratory Course for Reproductive Biology1 通年 農学研究科 2015/04-2016/03
生殖生物学専攻実験 Laboratory Course for Reproductive Biology 通年 農学研究科 2015/04-2016/03
生殖生物学演習１ Seminar for Reproductive Biology1 通年 農学研究科 2015/04-2016/03
生殖生物学演習 Seminar for Reproductive Biology 通年 農学研究科 2015/04-2016/03
細胞生物学Ⅱ Cell Biology II 前期 農学部 2015/04-2016/03
動物生理学 Physiology 前期 農学部 2016/04-2017/03
生殖生物学専攻実験１ Laboratory Course for Reproductive Biology1 通年 農学研究科 2016/04-2017/03
生殖生物学専攻実験２ Laboratory Course for Reproductive Biology2 通年 農学研究科 2016/04-2017/03
生殖生物学専攻実験 Laboratory Course for Reproductive Biology 通年 農学研究科 2016/04-2017/03
生殖生物学演習１ Seminar for Reproductive Biology1 通年 農学研究科 2016/04-2017/03
生殖生物学演習２ Seminar for Reproductive Biology2 通年 農学研究科 2016/04-2017/03
生殖生物学演習 Seminar for Reproductive Biology 通年 農学研究科 2016/04-2017/03
細胞生物学Ⅱ Cell Biology II 前期 農学部 2016/04-2017/03

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Supervising student clubs/circles
Club/circle name(Japanese) Club/circle name(English) Period
山歩会 1992/05/01-1994
School management (title, position)
Title Period
組換えDNA実験安全委員会委員 -
動物実験委員会委員 -
組換えDNA実験安全委員会 委員 2008/04/01-2011/03/31
動物実験委員会　外部評価導入ＷＧ 委員 2012/11/20-2013/10/10
Faculty management (title, position)
Title Period
国際交流委員会　委員 2013/04/01-2015/03/31
動物実験委員会　委員 2006/04/01-
環境・安全・衛生委員会　委員 -
Other activities (magazines and newspapers)
Title Contents Media Column Date
受精卵の発生左右する遺伝子の働き発見 京都新聞 教育欄 2015/07/25
体外受精の受胎確率判別 日経新聞 社会面 2015/06/30

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