中野雅博

Last Update: 2020/07/01 09:35:19

Name(Kanji/Kana/Abecedarium Latinum)

中野雅博/ナカノマサヒロ/Nakano, Masahiro

Primary Affiliation(Org1/Job title)

Institute for Frontier Life and Medical Sciences/Assistant Professor

Affiliated programs (koza)

Org1	Job title
Graduate Schools Biostudies	Assistant Professor

Academic Degree

Field(Japanese)	Field(English)	University(Japanese)	University(English)	Method
修士（農学）		九州大学
博士（農学）		九州大学

researchmap URL

https://researchmap.jp/7000015496

Research Topics

(Japanese)
インフルエンザウイルスのゲノム転写・複製機構の解明

Overview of the research

(Japanese)
インフルエンザウイルスのゲノムRNAは、核タンパク質NPやポリメラーゼとともにvRNPを形成する。vRNPはmRNA合成（転写）およびcRNA合成（複製の第一段階）を担う。ウイルス粒子から精製したvRNPは、柔軟な二重らせん構造を持つことが近年の電子顕微鏡解析より明らかとなっている。しかし、mRNAあるいはcRNA合成中のvRNPの構造については全く解明されておらず、また、これらのRNAがどのようにして合成されるのかも分かっていない。そこで私は、高速原子間力顕微鏡（AFM）を用いて機能的な状態のvRNPを解析することによって、微細構造学的観点からインフルエンザウイルスのゲノム転写・複製機構を明らかにすることを目的として研究を行っている。

(Japanese)

インフルエンザウイルスのゲノムRNAは、核タンパク質NPやポリメラーゼとともにvRNPを形成する。vRNPはmRNA合成（転写）およびcRNA合成（複製の第一段階）を担う。ウイルス粒子から精製したvRNPは、柔軟な二重らせん構造を持つことが近年の電子顕微鏡解析より明らかとなっている。しかし、mRNAあるいはcRNA合成中のvRNPの構造については全く解明されておらず、また、これらのRNAがどのようにして合成されるのかも分かっていない。そこで私は、高速原子間力顕微鏡（AFM）を用いて機能的な状態のvRNPを解析することによって、微細構造学的観点からインフルエンザウイルスのゲノム転写・複製機構を明らかにすることを目的として研究を行っている。

Fields of research (key words)

Key words(Japanese)	Key words(English)
ウイルス学
分子生物学

Published Papers

Author	Author(Japanese)	Author(English)	Title	Title(Japanese)	Title(English)	Bibliography	Bibliography(Japanese)	Bibliography(English)	Publication date	Refereed paper	Language	Publishing type	Disclose
Yutaro Yamagata, Yukiko Muramoto, Sho Miyamoto, Keiko Shindo, Masahiro Nakano, Takeshi Noda	Yutaro Yamagata, Yukiko Muramoto, Sho Miyamoto, Keiko Shindo, Masahiro Nakano, Takeshi Noda	Yutaro Yamagata, Yukiko Muramoto, Sho Miyamoto, Keiko Shindo, Masahiro Nakano, Takeshi Noda	Generation of a purely clonal defective interfering influenza virus.	Generation of a purely clonal defective interfering influenza virus.	Generation of a purely clonal defective interfering influenza virus.	Microbiology and immunology, 63, 5, 164-171	Microbiology and immunology, 63, 5, 164-171	Microbiology and immunology, 63, 5, 164-171	2019/05	Refereed	English		Disclose to all
Shimada T, Yamamoto K, Nakano M, Watanabe H, Schleheck D, Ishihama A	Shimada T, Yamamoto K, Nakano M, Watanabe H, Schleheck D, Ishihama A	Shimada T, Yamamoto K, Nakano M, Watanabe H, Schleheck D, Ishihama A	Regulatory role of CsqR (YihW) in transcription of the genes for catabolism of the anionic sugar sulfoquinovose (SQ) in Escherichia coli K-12.	Regulatory role of CsqR (YihW) in transcription of the genes for catabolism of the anionic sugar sulfoquinovose (SQ) in Escherichia coli K-12.	Regulatory role of CsqR (YihW) in transcription of the genes for catabolism of the anionic sugar sulfoquinovose (SQ) in Escherichia coli K-12.	Microbiology (Reading, England), 165, 1, 78-89	Microbiology (Reading, England), 165, 1, 78-89	Microbiology (Reading, England), 165, 1, 78-89	2019/01	Refereed			Disclose to all
Dai Hatakeyama, Masaki Shoji, Seiya Yamayoshi, Rina Yoh, Naho Ohmi, Shiori Takenaka, Ayaka Saitoh, Yumie Arakaki, Aki Masuda, Tsugunori Komatsu, Rina Nagano, Masahiro Nakano, Takeshi Noda, Yoshihiro Kawaoka, Takashi Kuzuhara	Dai Hatakeyama, Masaki Shoji, Seiya Yamayoshi, Rina Yoh, Naho Ohmi, Shiori Takenaka, Ayaka Saitoh, Yumie Arakaki, Aki Masuda, Tsugunori Komatsu, Rina Nagano, Masahiro Nakano, Takeshi Noda, Yoshihiro Kawaoka, Takashi Kuzuhara	Dai Hatakeyama, Masaki Shoji, Seiya Yamayoshi, Rina Yoh, Naho Ohmi, Shiori Takenaka, Ayaka Saitoh, Yumie Arakaki, Aki Masuda, Tsugunori Komatsu, Rina Nagano, Masahiro Nakano, Takeshi Noda, Yoshihiro Kawaoka, Takashi Kuzuhara	Influenza A virus nucleoprotein is acetylated by histone acetyltransferases PCAF and GCN5.	Influenza A virus nucleoprotein is acetylated by histone acetyltransferases PCAF and GCN5.	Influenza A virus nucleoprotein is acetylated by histone acetyltransferases PCAF and GCN5.	The Journal of biological chemistry, 293, 19, 7126-7138	The Journal of biological chemistry, 293, 19, 7126-7138	The Journal of biological chemistry, 293, 19, 7126-7138	2018/05/11	Refereed	English		Disclose to all
Sugino H, Usui T, Shimada T, Nakano M, Ogasawara H, Ishihama A, Hirata A	Sugino H, Usui T, Shimada T, Nakano M, Ogasawara H, Ishihama A, Hirata A	Sugino H, Usui T, Shimada T, Nakano M, Ogasawara H, Ishihama A, Hirata A	A structural sketch of RcdA, a transcription factor controlling the master regulator of biofilm formation.	A structural sketch of RcdA, a transcription factor controlling the master regulator of biofilm formation.	A structural sketch of RcdA, a transcription factor controlling the master regulator of biofilm formation.	FEBS letters	FEBS letters	FEBS letters	2017/06	Refereed			Disclose to all
Hiraku Takada, Tomohiro Shimada, Debashish Dey, M. Zuhaib Quyyum, Masahiro Nakano, Akira Ishiguro, Hideji Yoshida, Kaneyoshi Yamamoto, Ranjan Sen, Akira Ishihama	Hiraku Takada, Tomohiro Shimada, Debashish Dey, M. Zuhaib Quyyum, Masahiro Nakano, Akira Ishiguro, Hideji Yoshida, Kaneyoshi Yamamoto, Ranjan Sen, Akira Ishihama	Hiraku Takada, Tomohiro Shimada, Debashish Dey, M. Zuhaib Quyyum, Masahiro Nakano, Akira Ishiguro, Hideji Yoshida, Kaneyoshi Yamamoto, Ranjan Sen, Akira Ishihama	Differential Regulation of rRNA and tRNA Transcription from the rRNA-tRNA Composite Operon in Escherichia coli	Differential Regulation of rRNA and tRNA Transcription from the rRNA-tRNA Composite Operon in Escherichia coli	Differential Regulation of rRNA and tRNA Transcription from the rRNA-tRNA Composite Operon in Escherichia coli	PLoS One, 11, 12, e0163057	PLoS One, 11, 12, e0163057	PLoS One, 11, 12, e0163057	2016/12	Refereed	English	Research paper(scientific journal)	Disclose to all
Kaneyoshi Yamamoto, Masahiro Nakano, Akira Ishiharna	Kaneyoshi Yamamoto, Masahiro Nakano, Akira Ishiharna	Kaneyoshi Yamamoto, Masahiro Nakano, Akira Ishiharna	Regulatory role of transcription factor SutR (YdcN) in sulfur utilization in Escherichia coli	Regulatory role of transcription factor SutR (YdcN) in sulfur utilization in Escherichia coli	Regulatory role of transcription factor SutR (YdcN) in sulfur utilization in Escherichia coli	MICROBIOLOGY-SGM, 161, Pt 1, 99-111	MICROBIOLOGY-SGM, 161, Pt 1, 99-111	MICROBIOLOGY-SGM, 161, Pt 1, 99-111	2015/01	Refereed	English		Disclose to all
Masahiro Nakano, Kei Kamino	Masahiro Nakano, Kei Kamino	Masahiro Nakano, Kei Kamino	Amyloid-like Conformation and Interaction for the Self-Assembly in Barnacle Underwater Cement	Amyloid-like Conformation and Interaction for the Self-Assembly in Barnacle Underwater Cement	Amyloid-like Conformation and Interaction for the Self-Assembly in Barnacle Underwater Cement	BIOCHEMISTRY, 54, 3, 826-835	BIOCHEMISTRY, 54, 3, 826-835	BIOCHEMISTRY, 54, 3, 826-835	2015/01	Refereed	English	Research paper(scientific journal)	Disclose to all
Dai Hatakeyama, Masaki Shoji, Seiya Yamayoshi, Takenori Hirota, Monami Nagae, Shin Yanagisawa, Masahiro Nakano, Naho Ohmi, Takeshi Noda, Yoshihiro Kawaoka, Takashi Kuzuhara	Dai Hatakeyama, Masaki Shoji, Seiya Yamayoshi, Takenori Hirota, Monami Nagae, Shin Yanagisawa, Masahiro Nakano, Naho Ohmi, Takeshi Noda, Yoshihiro Kawaoka, Takashi Kuzuhara	Dai Hatakeyama, Masaki Shoji, Seiya Yamayoshi, Takenori Hirota, Monami Nagae, Shin Yanagisawa, Masahiro Nakano, Naho Ohmi, Takeshi Noda, Yoshihiro Kawaoka, Takashi Kuzuhara	A Novel Functional Site in the PB2 Subunit of Influenza A Virus Essential for Acetyl-CoA Interaction, RNA Polymerase Activity, and Viral Replication	A Novel Functional Site in the PB2 Subunit of Influenza A Virus Essential for Acetyl-CoA Interaction, RNA Polymerase Activity, and Viral Replication	A Novel Functional Site in the PB2 Subunit of Influenza A Virus Essential for Acetyl-CoA Interaction, RNA Polymerase Activity, and Viral Replication	JOURNAL OF BIOLOGICAL CHEMISTRY, 289, 36, 24980-24994	JOURNAL OF BIOLOGICAL CHEMISTRY, 289, 36, 24980-24994	JOURNAL OF BIOLOGICAL CHEMISTRY, 289, 36, 24980-24994	2014/09	Refereed	English	Research paper(scientific journal)	Disclose to all
Masahiro Nakano, Hiroshi Ogasawara, Tomohiro Shimada, Kaneyoshi Yamamoto, Akira Ishihama	Masahiro Nakano, Hiroshi Ogasawara, Tomohiro Shimada, Kaneyoshi Yamamoto, Akira Ishihama	Masahiro Nakano, Hiroshi Ogasawara, Tomohiro Shimada, Kaneyoshi Yamamoto, Akira Ishihama	Involvement of cAMP-CRP in transcription activation and repression of the pck gene encoding PEP carboxykinase, the key enzyme of gluconeogenesis	Involvement of cAMP-CRP in transcription activation and repression of the pck gene encoding PEP carboxykinase, the key enzyme of gluconeogenesis	Involvement of cAMP-CRP in transcription activation and repression of the pck gene encoding PEP carboxykinase, the key enzyme of gluconeogenesis	FEMS MICROBIOLOGY LETTERS, 355, 2, 93-99	FEMS MICROBIOLOGY LETTERS, 355, 2, 93-99	FEMS MICROBIOLOGY LETTERS, 355, 2, 93-99	2014/06	Refereed	English	Research paper(scientific journal)	Disclose to all
Tomohiro Shimada, Yasunori Katayama, Shuichi Kawakita, Hiroshi Ogasawara, Masahiro Nakano, Kaneyoshi Yamamoto, Akira Ishihama	Tomohiro Shimada, Yasunori Katayama, Shuichi Kawakita, Hiroshi Ogasawara, Masahiro Nakano, Kaneyoshi Yamamoto, Akira Ishihama	Tomohiro Shimada, Yasunori Katayama, Shuichi Kawakita, Hiroshi Ogasawara, Masahiro Nakano, Kaneyoshi Yamamoto, Akira Ishihama	A novel regulator RcdA of the csgD gene encoding the master regulator of biofilm formation in Escherichia coli	A novel regulator RcdA of the csgD gene encoding the master regulator of biofilm formation in Escherichia coli	A novel regulator RcdA of the csgD gene encoding the master regulator of biofilm formation in Escherichia coli	MICROBIOLOGYOPEN, 1, 4, 381-394	MICROBIOLOGYOPEN, 1, 4, 381-394	MICROBIOLOGYOPEN, 1, 4, 381-394	2012/12	Refereed	English	Research paper(scientific journal)	Disclose to all
Kei Kamino, Masahiro Nakano, Satoru Kanai	Kei Kamino, Masahiro Nakano, Satoru Kanai	Kei Kamino, Masahiro Nakano, Satoru Kanai	Significance of the conformation of building blocks in curing of barnacle underwater adhesive	Significance of the conformation of building blocks in curing of barnacle underwater adhesive	Significance of the conformation of building blocks in curing of barnacle underwater adhesive	FEBS JOURNAL, 279, 10, 1750-1760	FEBS JOURNAL, 279, 10, 1750-1760	FEBS JOURNAL, 279, 10, 1750-1760	2012/05	Refereed	English	Research paper(scientific journal)	Disclose to all
<< < > >> Title language:

Conference Activities & Talks

Title	Title(Japanese)	Title(English)	Conference	Conference(Japanese)	Conference(English)	Date	Language	Assortment	Disclose
Ultrastructure of the influenza virus ribonucleoprotein complexes producing viral RNAs	Ultrastructure of the influenza virus ribonucleoprotein complexes producing viral RNAs	Ultrastructure of the influenza virus ribonucleoprotein complexes producing viral RNAs	17th NSV 2018	17th NSV 2018	17th NSV 2018	2018/06	English	Oral presentation(general)	Disclose to all
AFM analysis of dsRNA binding mechanism of influenza A virus NS1	原子間力顕微鏡を用いたA型インフルエンザウイルスNS1の二本鎖RNAに対する結合機構の解析	AFM analysis of dsRNA binding mechanism of influenza A virus NS1	7th Negative Strand Virus-Japan Symposium	7th Negative Strand Virus-Japan Symposium	7th Negative Strand Virus-Japan Symposium	2018/01	Japanese	Oral presentation(general)	Disclose to all
Ultrastructural analysis of RNA synthesized by influenza virus vRNP	インフルエンザウイルスRNPが合成するRNAの微細構造解析	Ultrastructural analysis of RNA synthesized by influenza virus vRNP	RNAフロンティアミーティング2017	RNAフロンティアミーティング2017		2017/11	Japanese	Oral presentation(general)	Disclose to all
The mechanism of genome transcription and replication of influenza virus analyzed by high-speed atomic force microscopy	高速原子間力顕微鏡を用いたインフルエンザウイルスのゲノム転写・複製機構の解明	The mechanism of genome transcription and replication of influenza virus analyzed by high-speed atomic force microscopy	6th Negative Strand Virus-Japan Symposium	6th Negative Strand Virus-Japan Symposium	6th Negative Strand Virus-Japan Symposium	2017/01	Japanese	Oral presentation(general)	Disclose to all
Ultrastructural analysis of the influenza virus vRNP during transcription	転写中インフルエンザウイルスRNPの微細構造解析	Ultrastructural analysis of the influenza virus vRNP during transcription	5th Negative Strand Virus-Japan Symposium	5th Negative Strand Virus-Japan Symposium	5th Negative Strand Virus-Japan Symposium	2016/01	Japanese	Oral presentation(general)	Disclose to all
Analysis of influenza virus transcription using high-speed atomic force microscopy	高速原子間力顕微鏡を用いたインフルエンザウイルス転写機構の解析	Analysis of influenza virus transcription using high-speed atomic force microscopy	第29回インフルエンザ研究者交流の会シンポジウム	第29回インフルエンザ研究者交流の会シンポジウム		2015/05	Japanese	Oral presentation(general)	Disclose to all
Title language:

External funds: competitive funds and Grants-in-Aid for Scientific Research (Kakenhi)

Type	Position	Title(Japanese)	Period
基板研究(C)	Representative	高速原子間力顕微鏡を用いたウイルス転写複合体の微細構造解析	2016/04/01-2019/03/31
基盤研究(C)	Representative	高速原子間力顕微鏡を用いたウイルス転写複合体の微細構造解析（平成28年度分）	2016/04/01-2017/03/31
基盤研究(C)	Representative	高速原子間力顕微鏡を用いたウイルス転写複合体の微細構造解析（平成29年度分）	2017/04/01-2018/03/31
基盤研究(C)	Representative	高速原子間力顕微鏡を用いたウイルス転写複合体の微細構造解析（平成30年度分）	2018/04/01-2019/03/31
基盤研究(C)	Representative	インフルエンザウイルスタンパク質NS1の二本鎖RNAマスキング機構の解析（2019年度分）	2019/04/01-2020/03/31

Faculty management (title, position)

Title	Period
共同利用機器等委員会	2016/04/01-2017/03/31
化学物質管理委員会　委員	2016/04/01-2017/03/31
（旧ウイルス研）共同利用機器等委員会　委員	2016/10/01-2018/03/
化学物質管理委員会　委員	2016/10/01-
共同利用機器等委員会	2018/04/01-

中野 雅博

中野雅博